Lectin and cholera toxin binding to guinea pig tumor (104C1) cell surfaces before and after glycosphingolipid incorporation

M. Basu; S. Basu; W. G. Shanabruch; J. R. Moskal; C. H. Evans

doi:10.1016/0006-291X(76)90294-1

Lectin and cholera toxin binding to guinea pig tumor (104C1) cell surfaces before and after glycosphingolipid incorporation

M. Basu, S. Basu, W. G. Shanabruch, J. R. Moskal, C. H. Evans

Biomedical Engineering

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Abstract

¹²⁵I-Labeled Dolichos biflorus lectin and cholera toxin were used as probes for identification of Forssman- and GM1-type receptor sites on guinea pig tumor (104C1) cell surfaces. Increased binding of ¹²⁵I-labeled lectin and toxin to 104C1 cell surfaces was observed after the cells were treated with exogenous Forssman glycosphingolipid and GM1 ganglioside, respectively. Biosynthesis in vitro of these two glycosphingolipids from their precursor molecules was established using a membrane preparation isolated from confluent cultures of guinea pig tumor 104C1 cells.

Original language	English (US)
Pages (from-to)	385-392
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(76)90294-1
State	Published - Jul 12 1976

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "125I-Labeled Dolichos biflorus lectin and cholera toxin were used as probes for identification of Forssman- and GM1-type receptor sites on guinea pig tumor (104C1) cell surfaces. Increased binding of 125I-labeled lectin and toxin to 104C1 cell surfaces was observed after the cells were treated with exogenous Forssman glycosphingolipid and GM1 ganglioside, respectively. Biosynthesis in vitro of these two glycosphingolipids from their precursor molecules was established using a membrane preparation isolated from confluent cultures of guinea pig tumor 104C1 cells.",

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T1 - Lectin and cholera toxin binding to guinea pig tumor (104C1) cell surfaces before and after glycosphingolipid incorporation

AU - Basu, M.

AU - Basu, S.

AU - Shanabruch, W. G.

AU - Moskal, J. R.

AU - Evans, C. H.

PY - 1976/7/12

Y1 - 1976/7/12

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AB - 125I-Labeled Dolichos biflorus lectin and cholera toxin were used as probes for identification of Forssman- and GM1-type receptor sites on guinea pig tumor (104C1) cell surfaces. Increased binding of 125I-labeled lectin and toxin to 104C1 cell surfaces was observed after the cells were treated with exogenous Forssman glycosphingolipid and GM1 ganglioside, respectively. Biosynthesis in vitro of these two glycosphingolipids from their precursor molecules was established using a membrane preparation isolated from confluent cultures of guinea pig tumor 104C1 cells.

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Lectin and cholera toxin binding to guinea pig tumor (104C1) cell surfaces before and after glycosphingolipid incorporation

Abstract

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