Lectin and cholera toxin binding to guinea pig tumor (104C1) cell surfaces before and after glycosphingolipid incorporation

M. Basu, S. Basu, W. G. Shanabruch, J. R. Moskal, C. H. Evans

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

125I-Labeled Dolichos biflorus lectin and cholera toxin were used as probes for identification of Forssman- and GM1-type receptor sites on guinea pig tumor (104C1) cell surfaces. Increased binding of 125I-labeled lectin and toxin to 104C1 cell surfaces was observed after the cells were treated with exogenous Forssman glycosphingolipid and GM1 ganglioside, respectively. Biosynthesis in vitro of these two glycosphingolipids from their precursor molecules was established using a membrane preparation isolated from confluent cultures of guinea pig tumor 104C1 cells.

Original languageEnglish (US)
Pages (from-to)385-392
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume71
Issue number1
DOIs
StatePublished - Jul 12 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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