Lectin receptors on cells isolated from the turtle retina

P. Vijay Sarthy*, C. David Bridges, Francis L. Kretzer, Dominic M K Lam

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The presence of specific oligosaccharides on the surface of retinal cells was examined by incubating FITC‐labeled lectins with cells dissociated from papain‐treated turtle retinas. The pattern and intensity of binding was found to vary among the cells examined. With Con A, there was strong surface staining of both rods and cones, with an intense ring of fluorescence above the nucleus. The bipolar and ganglion cells also showed strong surface labeling. In Müller (glial) cells there was intense fluorescence in the apical, microvillous region. In contrast, the horizontal cells and their axons showed weak staining. When RCA‐60, RCA‐120, and WGA were incubated with photoreceptors, bipolar cells, or horizontal cells, little fluorescence was visible. However, all three lectins bound strongly to the Müller cells. In contrast, the Lotus lectin did not bind to any of the cells examined. In all the cases, lectin binding was inhibited by the appropriate haptene sugar. Further, prior treatment of cells with neuraminidase did not alter lectin binding to any cell type. These results suggest differences in the distribution of lectin receptors among specific cell types, and particularly between neurons and glial cells in the vertebrate retina.

Original languageEnglish (US)
Pages (from-to)561-569
Number of pages9
JournalJournal of Comparative Neurology
Volume202
Issue number4
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Neuroscience(all)

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