Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro

Hailei Yao; Xiaoyan Xie; Yanhua Li; Dongmei Wang; Shu Han; Shuangshuang Shi; Xue Nan; Cixian Bai; Yunfang Wang; Xuetao Pei

doi:10.1155/2008/531317

Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro

Hailei Yao, Xiaoyan Xie, Yanhua Li, Dongmei Wang, Shu Han, Shuangshuang Shi, Xue Nan, Cixian Bai, Yunfang Wang, Xuetao Pei^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.

Original language	English (US)
Article number	531317
Journal	Clinical and Developmental Immunology
Volume	2008
DOIs	https://doi.org/10.1155/2008/531317
State	Published - 2008
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro",

abstract = "In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.",

author = "Hailei Yao and Xiaoyan Xie and Yanhua Li and Dongmei Wang and Shu Han and Shuangshuang Shi and Xue Nan and Cixian Bai and Yunfang Wang and Xuetao Pei",

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doi = "10.1155/2008/531317",

language = "English (US)",

volume = "2008",

journal = "Clinical and Developmental Immunology",

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T1 - Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro

AU - Yao, Hailei

AU - Xie, Xiaoyan

AU - Li, Yanhua

AU - Wang, Dongmei

AU - Han, Shu

AU - Shi, Shuangshuang

AU - Nan, Xue

AU - Bai, Cixian

AU - Wang, Yunfang

AU - Pei, Xuetao

PY - 2008

Y1 - 2008

N2 - In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.

AB - In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.

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DO - 10.1155/2008/531317

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JO - Clinical and Developmental Immunology

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Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro

Abstract

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