Leucocyte labeling with colloidal 111In in whole blood

A Michael Zimmer*, Stewart M Spies

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A simple method of 111In leucocyte labeling in whole blood is described. 111In-colloid is initially formulated using iron (50 μg Fe+3). 111In-colloid is then added to 10 mL of whole blood and leucocyte labeling is achieved by phagocytosis. Non-phagocytosed 111In-colloid is solubilized using a sodium citrate solution. Labeling efficiencies of 60-80% have been achieved with this method. If needed, excess 111In-soluble-complexes can be removed in the plasma fraction by appropriate centrifugation. The labeling method described is easy to use and has the advantage of selectively labeling leucocytes in a whole blood mixture.

Original languageEnglish (US)
Pages (from-to)1541-1544
Number of pages4
JournalThe International Journal Of Applied Radiation And Isotopes
Volume34
Issue number11
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Radiation
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging

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