A simple method of 111In leucocyte labeling in whole blood is described. 111In-colloid is initially formulated using iron (50 μg Fe+3). 111In-colloid is then added to 10 mL of whole blood and leucocyte labeling is achieved by phagocytosis. Non-phagocytosed 111In-colloid is solubilized using a sodium citrate solution. Labeling efficiencies of 60-80% have been achieved with this method. If needed, excess 111In-soluble-complexes can be removed in the plasma fraction by appropriate centrifugation. The labeling method described is easy to use and has the advantage of selectively labeling leucocytes in a whole blood mixture.
|Original language||English (US)|
|Number of pages||4|
|Journal||The International Journal Of Applied Radiation And Isotopes|
|State||Published - Jan 1 1983|
ASJC Scopus subject areas
- Nuclear Energy and Engineering
- Radiology Nuclear Medicine and imaging