TY - JOUR
T1 - Leukotriene B4 as a mediator of early and late reactions to antigen in humans
T2 - The effect of systemic glucocorticoid treatment in vivo
AU - Freeland, Howard S.
AU - Pipkorn, Ulf
AU - Schleimer, Robert P.
AU - Bascom, Rebecca
AU - Lichtenstein, Lawrence M.
AU - Naclerio, Robert M.
AU - Peters, Stephen P.
N1 - Funding Information:
From the Department of Medicine, Division of Clinical Immunol-ogy, Johns Hopkins University School of Medicine, Baltimore, Md., and the Department of Medicine, Division of Pulmonary Medicine and Critical Care, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa. Supported in part by National Institutes of Health Grants AI-24509, A&20136, AR31891, NS-22488, and A&08270. Received for publication May 6, 1988. Accepted for publication Aug. 17, 1988. Reprint requests: Stephen P. Peters, The Jefferson Medical College, Division of Pulmonary and Critical Care Medicine, 804 College Building, 1025 Walnut St., Philadelphia, PA 19107.
Funding Information:
Dr. Pipkorn is a recipient of an International Research Fellowship Award from the Fogerty International Center, National Institutes of Health (F05 TWO 351601).
PY - 1989/3
Y1 - 1989/3
N2 - The role played by leukotriene B4 (LTB4) in human allergic reactions has been the subject of recent interest and speculation. To characterize further the role of this mediator, we quantitated LTB4 levels in nasal washing by radioimmunoassay in 13 atopic subjects during immediate and late reactions after nasal antigen challenge while the subjects were taking placebo or prednisone (20 mg every 8 hours for 48 hours) in a double-blind, placebo-controlled, crossover protocol and compared these levels with levels of seven nonatopic subjects undergoing similar nasal antigen challenge. Nasal antigen challenge of atopic subjects resulted in an increase in LTB4 levels during the immediate reaction in 10 of 13 subjects ( 9 13 with a >50% increase over baseline) with no similar increase observed in nonatopic subjects (p < 0.05). An increase in LTB4 levels was observed in 12 13 atopic subjects ( 6 13 with >50% increase over a second baseline) during late time periods (p < 0.05), which was associated with an influx of neutrophils (from 65,000 ± 43,000 to 1,246,000 ± 829,000; p < 0.05). However, nonatopic subjects appeared to demonstrate a similar late increase in LTB4 levels. High-performance liquid chromatography analysis of immunoreactive LTB4 demonstrated that 84% of immunoreactive LTB4 coeluted with the biologically isomer during the immediate reaction, whereas 44% to 61% coeluted with the biologically active isomer during late reactions. Steroid pretreatment had no effect on either the early or late increase in LTB4 levels or on the neutrophil influx observed during the late reaction. In a second series of studies involving eight subjects, we demonstrated that a single 50 mg oral dose of prednisone had no effect on the release of LTB4 from neutrophils stimulated ex vivo with calcium ionophore A23187 or serum-activated zymosan, and, furthermore, that neutrophil concentration ("density") had a marked effect on the ability of neutrophils to metabolize LTB4 that they synthesize. These results indicate that human allergic nasal reactions are associated with the release of LTB4 in vivo and that these levels and neutrophil influx are not modified by systemic steroid pretreatment. Because multiple inflammatory cells may participate in these allergic reactions to synthesize and metabolize LTB4, this mediator may play a complex role in human allergic reactions.
AB - The role played by leukotriene B4 (LTB4) in human allergic reactions has been the subject of recent interest and speculation. To characterize further the role of this mediator, we quantitated LTB4 levels in nasal washing by radioimmunoassay in 13 atopic subjects during immediate and late reactions after nasal antigen challenge while the subjects were taking placebo or prednisone (20 mg every 8 hours for 48 hours) in a double-blind, placebo-controlled, crossover protocol and compared these levels with levels of seven nonatopic subjects undergoing similar nasal antigen challenge. Nasal antigen challenge of atopic subjects resulted in an increase in LTB4 levels during the immediate reaction in 10 of 13 subjects ( 9 13 with a >50% increase over baseline) with no similar increase observed in nonatopic subjects (p < 0.05). An increase in LTB4 levels was observed in 12 13 atopic subjects ( 6 13 with >50% increase over a second baseline) during late time periods (p < 0.05), which was associated with an influx of neutrophils (from 65,000 ± 43,000 to 1,246,000 ± 829,000; p < 0.05). However, nonatopic subjects appeared to demonstrate a similar late increase in LTB4 levels. High-performance liquid chromatography analysis of immunoreactive LTB4 demonstrated that 84% of immunoreactive LTB4 coeluted with the biologically isomer during the immediate reaction, whereas 44% to 61% coeluted with the biologically active isomer during late reactions. Steroid pretreatment had no effect on either the early or late increase in LTB4 levels or on the neutrophil influx observed during the late reaction. In a second series of studies involving eight subjects, we demonstrated that a single 50 mg oral dose of prednisone had no effect on the release of LTB4 from neutrophils stimulated ex vivo with calcium ionophore A23187 or serum-activated zymosan, and, furthermore, that neutrophil concentration ("density") had a marked effect on the ability of neutrophils to metabolize LTB4 that they synthesize. These results indicate that human allergic nasal reactions are associated with the release of LTB4 in vivo and that these levels and neutrophil influx are not modified by systemic steroid pretreatment. Because multiple inflammatory cells may participate in these allergic reactions to synthesize and metabolize LTB4, this mediator may play a complex role in human allergic reactions.
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U2 - 10.1016/0091-6749(89)90076-6
DO - 10.1016/0091-6749(89)90076-6
M3 - Article
C2 - 2466880
AN - SCOPUS:0024478292
SN - 0091-6749
VL - 83
SP - 634
EP - 642
JO - The Journal of allergy and clinical immunology
JF - The Journal of allergy and clinical immunology
IS - 3
ER -