LeY oligosaccharide upregulates DAG/PKC signaling pathway in the human endometrial cells

Yali Li, Keli Ma, Ping Sun, Shuai Liu, Huamin Qin, Zhengmei Zhu, Xiaoqi Wang, Qiu Yan*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

LeY oligosaccharide is stage specifically expressed by the embryo and uterine endometrium, and it plays important roles in embryo implantation. In addition to participating in the recognition and adhesion on fetal-maternal interface, LeY potentially regulates the expression of some implantation-related factors. However, it remains elusive whether it can mediate the involved signaling pathway. In this study, agarose-LeY beads were used to mimic the embryos, and the effects of LeY oligosaccharide on DAG/PKC signaling pathway was studied in human endometrial epithelial cells. Results showed that LeY could significantly trigger the activation of cPKCα and cPKCbβ2, and their translocation from the cytosol to the plasma membrane. The cellular DAG content was also upregulated, and the activation of PLCγ1 was promoted. On the contrary, DAG/PKC signaling pathway was significantly inhibited when anti-LeY antibody was used after confirmation of LeY expression in human endometrial epithelial cells by immunohistochemistry and flow cytometry. These results suggest that LeY oligosaccharide acts as a signal molecule to modulate DAG/PKC signaling pathway.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume331
Issue number1-2
DOIs
StatePublished - May 18 2009

Keywords

  • DAG
  • Endometrial cells
  • LeY oligosaccharide
  • PKC PLCγ

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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