Abstract
LeY oligosaccharide is stage specifically expressed by the embryo and uterine endometrium, and it plays important roles in embryo implantation. In addition to participating in the recognition and adhesion on fetal-maternal interface, LeY potentially regulates the expression of some implantation-related factors. However, it remains elusive whether it can mediate the involved signaling pathway. In this study, agarose-LeY beads were used to mimic the embryos, and the effects of LeY oligosaccharide on DAG/PKC signaling pathway was studied in human endometrial epithelial cells. Results showed that LeY could significantly trigger the activation of cPKCα and cPKCbβ2, and their translocation from the cytosol to the plasma membrane. The cellular DAG content was also upregulated, and the activation of PLCγ1 was promoted. On the contrary, DAG/PKC signaling pathway was significantly inhibited when anti-LeY antibody was used after confirmation of LeY expression in human endometrial epithelial cells by immunohistochemistry and flow cytometry. These results suggest that LeY oligosaccharide acts as a signal molecule to modulate DAG/PKC signaling pathway.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-7 |
| Number of pages | 7 |
| Journal | Molecular and Cellular Biochemistry |
| Volume | 331 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 2009 |
Funding
Acknowledgments The authors would like to thank Professor Sen-ITIROH Hakomori for providing anti-LeY antibody, Professor Zhongming Wu for contributing the human endometrial cell line and Gan Hui Theng for technical support. This work was supported by grants from the National Natural Science Foundation of China (No: 30270329 and 30670465).
Keywords
- DAG
- Endometrial cells
- LeY oligosaccharide
- PKC PLCγ
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology