LIF-and IL-1β-mediated increases in substance P receptor mRNA in axotomized, explanted or dissociated sympathetic ganglia

William H. Ludlam*, Karen J. Chandross, John A. Kessler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Regulation of substance P receptor (SPR) mRNA examined in the rat sympathetic superior cervical ganglion (SCG) in vitro and in vivo after axotomy. Interleukin-1β (IL-1β) treatment of explanted ganglia elevated levels of SPR mRNA. By contrast, dissociated cultures of purified sympathetic neurons, purified Schwann cells each expressed only low levels of SPR mRNA, and treatment with the cytokine did not alter levels of the receptor mRNA. Treatment of Schwann cell or fibroblast cultures with leukemia inhibitory factor (LIF) also did not alter SPR mRNA. However, treatment of pure neuronal cultures with LIF significantly elevated levels of the receptor mRNA. Further, SPR mRNA increased in pure sympathetic neurons cultured in the presence of conditioned medium from IL-1β treated fibroblasts or Schwann cells; this effect was blocked in the presence of LIF antibody. This suggests that the stimulatory effects of IL-1β on SPR mRNA in explants is mediated by LIF release. Axotomy of the SCG in vivo resulted in a significant increase in LIF mRNA. Further, axotomy resulted in a significant increase in SPR mRNA, suggesting that LIF may mediate the increase in SPR mRNA. In view of the known effects of substance P (SP) on inflammatory responses, these observations suggest that coordinated expression of SP and SPR mRNA in neurons after nerve injury may participate in inflammatory and repair processes in the ganglion.

Original languageEnglish (US)
Pages (from-to)12-20
Number of pages9
JournalBrain research
Volume685
Issue number1-2
DOIs
StatePublished - Jul 10 1995

Keywords

  • Axotomy
  • Fibroblast
  • IL-1β
  • LIF
  • Nuclease protection assay
  • Receptor
  • Regulation
  • Schwann cell
  • Substance P
  • Sympathetic
  • mRNA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'LIF-and IL-1β-mediated increases in substance P receptor mRNA in axotomized, explanted or dissociated sympathetic ganglia'. Together they form a unique fingerprint.

Cite this