Ligand migration pathway and protein dynamics in myoglobin: A time-resolved crystallographic study on L29W MbCO

Marius Schmidt*, Karin Nienhaus, Reinhard Pahl, Angela Krasselt, Spencer Anderson, Fritz Parak, G. Ulrich Nienhaus, Vukica Šrajer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

136 Scopus citations

Abstract

By using time-resolved x-ray crystallography at room temperature, structural relaxations and ligand migration were examined in myoglobin (Mb) mutant L29W from nanoseconds to seconds after photodissociation of carbon monoxide (CO) from the heme iron by nanosecond laser pulses, The data were analyzed in terms of transient kinetics by fitting trial functions to integrated difference electron density values obtained from select structural moieties, thus allowing a quantitative description of the processes involved. The observed relaxations are linked to other investigations on protein dynamics, At the earliest times, the heme has already completely relaxed into its domed deoxy structure, and there is no photodissociated CO visible at the primary docking site, Initial relaxations of larger globin moieties are completed within several hundred nanoseconds. They influence the concomitant migration of photodissociated CO to the Xe1 site, where it appears at ≈300 ns and leaves again at ≈1.5 ms. The extremely long residence time in Xe1 as compared with wild-type MbCO implies that, in the latter protein, the CO exits the protein from Xe1 predominantly via the distal pocket, A well-defined deligated state is populated between ≈2 μs and ≈1 ms; its structure is very similar to the equilibrium deoxy structure, Between 1.5 and 20 ms, no CO is visible in the protein interior; it is either distributed among many sites within the protein or has escaped to the solvent, Finally, recombination at the heme iron occurs after >20 ms.

Original languageEnglish (US)
Pages (from-to)11704-11709
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number33
DOIs
StatePublished - Aug 16 2005

Keywords

  • Kinetics
  • Laue crystallography
  • Protein relaxation

ASJC Scopus subject areas

  • General

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