Ligand-specific control of src-suppressed C kinase substrate gene expression

Stephen R. Coats*, Lil M. Pabón-Pea, Joseph W. Covington, Douglas E. Vaughan

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The src-suppressed C-kinase substrate, SSeCKS, is now recognized as a key regulator of cell signaling and cytoskeletal dynamics. However, few ligands that control SSeCKS expression have been identified. We report that platelet-derived growth factor-BB (PDGF-BB), lysophosphatidic acid (LPA), and eicosapentaenoic acid (EPA) potently modulate SSeCKS gene expression in cultured smooth muscle (RASM) cells relative to other bioactive ligands tested. In addition, EPA-dependent regulation of SSeCKS expression correlates with distinct changes in cell morphology and adhesion in RASM cells. Independent evidence that ligand-specific control of SSeCKS expression links to the regulation of cell adhesion and morphology was obtained using ras-transformed fibroblasts, KNRK. Sodium butyrate (NaB) upregulates SSeCKS mRNA and protein expression corresponding to increased cell-spreading and adhesion. In addition, ectopic expression of recombinant SSeCKS recapitulates attributes of NaB-induced morphogenesis in KNRK cells. The data provide novel evidence that SSeCKS functions in PDGF-BB-, LPA-, EPA-, and NaB-mediated cell signaling.

Original languageEnglish (US)
Pages (from-to)1112-1120
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number5
DOIs
StatePublished - Nov 6 2002

Keywords

  • Adhesion
  • EPA
  • LPA
  • Morphology
  • NaB
  • PDGF-BB
  • SSeCKS

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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