Ligand-specific control of src-suppressed C kinase substrate gene expression

Stephen R. Coats; Lil M. Pabón-Pea; Joseph W. Covington; Douglas E. Vaughan

doi:10.1016/S0006-291X(02)02275-1

Ligand-specific control of src-suppressed C kinase substrate gene expression

Stephen R. Coats^*, Lil M. Pabón-Pea, Joseph W. Covington, Douglas E. Vaughan

^*Corresponding author for this work

Medicine, Cardiology Division

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The src-suppressed C-kinase substrate, SSeCKS, is now recognized as a key regulator of cell signaling and cytoskeletal dynamics. However, few ligands that control SSeCKS expression have been identified. We report that platelet-derived growth factor-BB (PDGF-BB), lysophosphatidic acid (LPA), and eicosapentaenoic acid (EPA) potently modulate SSeCKS gene expression in cultured smooth muscle (RASM) cells relative to other bioactive ligands tested. In addition, EPA-dependent regulation of SSeCKS expression correlates with distinct changes in cell morphology and adhesion in RASM cells. Independent evidence that ligand-specific control of SSeCKS expression links to the regulation of cell adhesion and morphology was obtained using ras-transformed fibroblasts, KNRK. Sodium butyrate (NaB) upregulates SSeCKS mRNA and protein expression corresponding to increased cell-spreading and adhesion. In addition, ectopic expression of recombinant SSeCKS recapitulates attributes of NaB-induced morphogenesis in KNRK cells. The data provide novel evidence that SSeCKS functions in PDGF-BB-, LPA-, EPA-, and NaB-mediated cell signaling.

Original language	English (US)
Pages (from-to)	1112-1120
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	297
Issue number	5
DOIs	https://doi.org/10.1016/S0006-291X(02)02275-1
State	Published - 2002

Keywords

Adhesion
EPA
LPA
Morphology
NaB
PDGF-BB
SSeCKS

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(02)02275-1

Cite this

@article{a73def0736194f119f24470f2b132e00,

title = "Ligand-specific control of src-suppressed C kinase substrate gene expression",

abstract = "The src-suppressed C-kinase substrate, SSeCKS, is now recognized as a key regulator of cell signaling and cytoskeletal dynamics. However, few ligands that control SSeCKS expression have been identified. We report that platelet-derived growth factor-BB (PDGF-BB), lysophosphatidic acid (LPA), and eicosapentaenoic acid (EPA) potently modulate SSeCKS gene expression in cultured smooth muscle (RASM) cells relative to other bioactive ligands tested. In addition, EPA-dependent regulation of SSeCKS expression correlates with distinct changes in cell morphology and adhesion in RASM cells. Independent evidence that ligand-specific control of SSeCKS expression links to the regulation of cell adhesion and morphology was obtained using ras-transformed fibroblasts, KNRK. Sodium butyrate (NaB) upregulates SSeCKS mRNA and protein expression corresponding to increased cell-spreading and adhesion. In addition, ectopic expression of recombinant SSeCKS recapitulates attributes of NaB-induced morphogenesis in KNRK cells. The data provide novel evidence that SSeCKS functions in PDGF-BB-, LPA-, EPA-, and NaB-mediated cell signaling.",

keywords = "Adhesion, EPA, LPA, Morphology, NaB, PDGF-BB, SSeCKS",

author = "Coats, {Stephen R.} and Pab{\'o}n-Pea, {Lil M.} and Covington, {Joseph W.} and Vaughan, {Douglas E.}",

note = "Funding Information: This work was supported by NIHLBI R01 #51387 to Douglas E. Vaughan. We thank Myrtle and Robert Griffiths, and J. Brian Robertson for assistance with manuscript preparation.",

year = "2002",

doi = "10.1016/S0006-291X(02)02275-1",

language = "English (US)",

volume = "297",

pages = "1112--1120",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "5",

}

TY - JOUR

T1 - Ligand-specific control of src-suppressed C kinase substrate gene expression

AU - Coats, Stephen R.

AU - Pabón-Pea, Lil M.

AU - Covington, Joseph W.

AU - Vaughan, Douglas E.

N1 - Funding Information: This work was supported by NIHLBI R01 #51387 to Douglas E. Vaughan. We thank Myrtle and Robert Griffiths, and J. Brian Robertson for assistance with manuscript preparation.

PY - 2002

Y1 - 2002

N2 - The src-suppressed C-kinase substrate, SSeCKS, is now recognized as a key regulator of cell signaling and cytoskeletal dynamics. However, few ligands that control SSeCKS expression have been identified. We report that platelet-derived growth factor-BB (PDGF-BB), lysophosphatidic acid (LPA), and eicosapentaenoic acid (EPA) potently modulate SSeCKS gene expression in cultured smooth muscle (RASM) cells relative to other bioactive ligands tested. In addition, EPA-dependent regulation of SSeCKS expression correlates with distinct changes in cell morphology and adhesion in RASM cells. Independent evidence that ligand-specific control of SSeCKS expression links to the regulation of cell adhesion and morphology was obtained using ras-transformed fibroblasts, KNRK. Sodium butyrate (NaB) upregulates SSeCKS mRNA and protein expression corresponding to increased cell-spreading and adhesion. In addition, ectopic expression of recombinant SSeCKS recapitulates attributes of NaB-induced morphogenesis in KNRK cells. The data provide novel evidence that SSeCKS functions in PDGF-BB-, LPA-, EPA-, and NaB-mediated cell signaling.

AB - The src-suppressed C-kinase substrate, SSeCKS, is now recognized as a key regulator of cell signaling and cytoskeletal dynamics. However, few ligands that control SSeCKS expression have been identified. We report that platelet-derived growth factor-BB (PDGF-BB), lysophosphatidic acid (LPA), and eicosapentaenoic acid (EPA) potently modulate SSeCKS gene expression in cultured smooth muscle (RASM) cells relative to other bioactive ligands tested. In addition, EPA-dependent regulation of SSeCKS expression correlates with distinct changes in cell morphology and adhesion in RASM cells. Independent evidence that ligand-specific control of SSeCKS expression links to the regulation of cell adhesion and morphology was obtained using ras-transformed fibroblasts, KNRK. Sodium butyrate (NaB) upregulates SSeCKS mRNA and protein expression corresponding to increased cell-spreading and adhesion. In addition, ectopic expression of recombinant SSeCKS recapitulates attributes of NaB-induced morphogenesis in KNRK cells. The data provide novel evidence that SSeCKS functions in PDGF-BB-, LPA-, EPA-, and NaB-mediated cell signaling.

KW - Adhesion

KW - EPA

KW - LPA

KW - Morphology

KW - NaB

KW - PDGF-BB

KW - SSeCKS

UR - http://www.scopus.com/inward/record.url?scp=0036401167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036401167&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(02)02275-1

DO - 10.1016/S0006-291X(02)02275-1

M3 - Article

C2 - 12372401

AN - SCOPUS:0036401167

SN - 0006-291X

VL - 297

SP - 1112

EP - 1120

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 5

ER -

Ligand-specific control of src-suppressed C kinase substrate gene expression

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this