Light-induced FTIR difference spectroscopy of the S2-to-S3 state transition of the oxygen-evolving complex in Photosystem II

Hsiu An Chu, Warwick Hillier, Neil A. Law, Heather Sackett, Shannon Haymond, Gerald T. Babcock*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We have applied flash-induced FTIR spectroscopy to study structural changes upon the S2-to-S3 state transition of the oxygen-evolving complex (OEC) in Photosystem II (PSII). We found that several modes in the difference IR spectrum are associated with bond rearrangements induced by the second laser flash. Most of these IR modes are absent in spectra of S2/S1, of the acceptor-side non-heme ion, of Y.(D)/Y(D) and of S3'/S2' from Ca-depleted PSII preparations. Our results suggest that these IR modes most likely originate from structural changes in the oxygen-evolving complex itself upon the S2-to-S3 state transition in PSII. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)528-532
Number of pages5
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1459
Issue number2-3
DOIs
StatePublished - Aug 15 2000

Keywords

  • Fourier transform infrared spectroscopy
  • Manganese cluster
  • Oxygen evolution
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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