Light-induced FTIR difference spectroscopy of the S2-to-S3 state transition of the oxygen-evolving complex in Photosystem II

Hsiu An Chu; Warwick Hillier; Neil A. Law; Heather Sackett; Shannon Haymond; Gerald T. Babcock

doi:10.1016/S0005-2728(00)00193-6

Light-induced FTIR difference spectroscopy of the S₂-to-S₃ state transition of the oxygen-evolving complex in Photosystem II

Hsiu An Chu, Warwick Hillier, Neil A. Law, Heather Sackett, Shannon Haymond, Gerald T. Babcock^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We have applied flash-induced FTIR spectroscopy to study structural changes upon the S₂-to-S₃ state transition of the oxygen-evolving complex (OEC) in Photosystem II (PSII). We found that several modes in the difference IR spectrum are associated with bond rearrangements induced by the second laser flash. Most of these IR modes are absent in spectra of S₂/S₁, of the acceptor-side non-heme ion, of Y^.(D)/Y(D) and of S₃'/S₂' from Ca-depleted PSII preparations. Our results suggest that these IR modes most likely originate from structural changes in the oxygen-evolving complex itself upon the S₂-to-S₃ state transition in PSII. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	528-532
Number of pages	5
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1459
Issue number	2-3
DOIs	https://doi.org/10.1016/S0005-2728(00)00193-6
State	Published - Aug 15 2000

Keywords

Fourier transform infrared spectroscopy
Manganese cluster
Oxygen evolution
Photosystem II

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/S0005-2728(00)00193-6

Cite this

@article{f65f686562ea418181583c013673dad4,

title = "Light-induced FTIR difference spectroscopy of the S2-to-S3 state transition of the oxygen-evolving complex in Photosystem II",

abstract = "We have applied flash-induced FTIR spectroscopy to study structural changes upon the S2-to-S3 state transition of the oxygen-evolving complex (OEC) in Photosystem II (PSII). We found that several modes in the difference IR spectrum are associated with bond rearrangements induced by the second laser flash. Most of these IR modes are absent in spectra of S2/S1, of the acceptor-side non-heme ion, of Y.(D)/Y(D) and of S3'/S2' from Ca-depleted PSII preparations. Our results suggest that these IR modes most likely originate from structural changes in the oxygen-evolving complex itself upon the S2-to-S3 state transition in PSII. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Fourier transform infrared spectroscopy, Manganese cluster, Oxygen evolution, Photosystem II",

author = "Chu, {Hsiu An} and Warwick Hillier and Law, {Neil A.} and Heather Sackett and Shannon Haymond and Babcock, {Gerald T.}",

note = "Funding Information: The authors wish to thank the funding by a National Institute of Health grant to G.T.B. (GM37300) and by the USDA-CRGO.",

year = "2000",

month = aug,

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doi = "10.1016/S0005-2728(00)00193-6",

language = "English (US)",

volume = "1459",

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TY - JOUR

T1 - Light-induced FTIR difference spectroscopy of the S2-to-S3 state transition of the oxygen-evolving complex in Photosystem II

AU - Chu, Hsiu An

AU - Hillier, Warwick

AU - Law, Neil A.

AU - Sackett, Heather

AU - Haymond, Shannon

AU - Babcock, Gerald T.

N1 - Funding Information: The authors wish to thank the funding by a National Institute of Health grant to G.T.B. (GM37300) and by the USDA-CRGO.

PY - 2000/8/15

Y1 - 2000/8/15

N2 - We have applied flash-induced FTIR spectroscopy to study structural changes upon the S2-to-S3 state transition of the oxygen-evolving complex (OEC) in Photosystem II (PSII). We found that several modes in the difference IR spectrum are associated with bond rearrangements induced by the second laser flash. Most of these IR modes are absent in spectra of S2/S1, of the acceptor-side non-heme ion, of Y.(D)/Y(D) and of S3'/S2' from Ca-depleted PSII preparations. Our results suggest that these IR modes most likely originate from structural changes in the oxygen-evolving complex itself upon the S2-to-S3 state transition in PSII. Copyright (C) 2000 Elsevier Science B.V.

AB - We have applied flash-induced FTIR spectroscopy to study structural changes upon the S2-to-S3 state transition of the oxygen-evolving complex (OEC) in Photosystem II (PSII). We found that several modes in the difference IR spectrum are associated with bond rearrangements induced by the second laser flash. Most of these IR modes are absent in spectra of S2/S1, of the acceptor-side non-heme ion, of Y.(D)/Y(D) and of S3'/S2' from Ca-depleted PSII preparations. Our results suggest that these IR modes most likely originate from structural changes in the oxygen-evolving complex itself upon the S2-to-S3 state transition in PSII. Copyright (C) 2000 Elsevier Science B.V.

KW - Fourier transform infrared spectroscopy

KW - Manganese cluster

KW - Oxygen evolution

KW - Photosystem II

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