Light scattering spectroscopic imaging of tissues

V. Backman*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

Abstract

Light scattering spectroscopic (LSS) imaging is a novel optical technology developed to probe the structure of living cells and tissues without need for tissue processing such as fixation or staining. LSS makes it possible to significantly enhance single backscattering from cell components such as nuclei and other organelles and differentiate this component from multiply scattered light. The spectrum of the single backscattering is further analyzed to provide quantitative information about cell morphology such as nuclear size, degree of pleomorphism, degree of hyperchromasia, amount of chromatin, nuclear and cytoplasmic sub-micron texture, etc. In LSS imaging, each pixel of a 2D-image is represented by a spectrum of backscattered light and a map of histological properties over wide areas of tissue is obtained. Because alteration of cell and nuclear morphology are associated with precancerous and cancerous changes in the majority of epithelia, LSS imaging can be used to detect precancerous lesions in optically accessible organs.

Original languageEnglish (US)
Pages (from-to)2316-2318
Number of pages3
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume3
StatePublished - 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Keywords

  • Cancer
  • Imaging
  • Scattering
  • Spectroscopy

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Fingerprint

Dive into the research topics of 'Light scattering spectroscopic imaging of tissues'. Together they form a unique fingerprint.

Cite this