Lineage plasticity and determinism in ex vivo differentiation of hematopoietic stem cells examined by large-scale transcriptional analysis

L. T. Huang; C. Chen; E. T. Papoutsakis; W. M. Miller

Lineage plasticity and determinism in ex vivo differentiation of hematopoietic stem cells examined by large-scale transcriptional analysis

L. T. Huang^*, C. Chen, E. T. Papoutsakis, W. M. Miller

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

The transcriptional programs of human hematopoietic stem cells (HSC) and early G and Mk differentiation grom human HSC in response to lineage-specific cytokines were studied by utilizing DNA microarrays containing over 21,000 human genes. The degree of respective lineage comitments were assessed by detection of lineage associated surface markers using flow cytometry. The rsults show the decrease in expression of HSC realted genes and proteins in G and Mk cultures. In Mk cells, the expression levels of granule proteins myeloperoxidase (MPO), cathepsin G (CTSG) and Charot-Leyden crystal protein (CLC) were comparable to or higher than those in G cells. G-cells displayed a high mRNA level of GATA-1, an important Mk transcription factor.

Original language	English (US)
Pages	8341
Number of pages	1
State	Published - 2004
Event	2004 AIChE Annual Meeting - Austin, TX, United States Duration: Nov 7 2004 → Nov 12 2004

Other

Other	2004 AIChE Annual Meeting
Country/Territory	United States
City	Austin, TX
Period	11/7/04 → 11/12/04

ASJC Scopus subject areas

General Engineering

Cite this

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title = "Lineage plasticity and determinism in ex vivo differentiation of hematopoietic stem cells examined by large-scale transcriptional analysis",

abstract = "The transcriptional programs of human hematopoietic stem cells (HSC) and early G and Mk differentiation grom human HSC in response to lineage-specific cytokines were studied by utilizing DNA microarrays containing over 21,000 human genes. The degree of respective lineage comitments were assessed by detection of lineage associated surface markers using flow cytometry. The rsults show the decrease in expression of HSC realted genes and proteins in G and Mk cultures. In Mk cells, the expression levels of granule proteins myeloperoxidase (MPO), cathepsin G (CTSG) and Charot-Leyden crystal protein (CLC) were comparable to or higher than those in G cells. G-cells displayed a high mRNA level of GATA-1, an important Mk transcription factor.",

author = "Huang, {L. T.} and C. Chen and Papoutsakis, {E. T.} and Miller, {W. M.}",

year = "2004",

language = "English (US)",

pages = "8341",

note = "2004 AIChE Annual Meeting ; Conference date: 07-11-2004 Through 12-11-2004",

}

TY - CONF

T1 - Lineage plasticity and determinism in ex vivo differentiation of hematopoietic stem cells examined by large-scale transcriptional analysis

AU - Huang, L. T.

AU - Chen, C.

AU - Papoutsakis, E. T.

AU - Miller, W. M.

PY - 2004

Y1 - 2004

N2 - The transcriptional programs of human hematopoietic stem cells (HSC) and early G and Mk differentiation grom human HSC in response to lineage-specific cytokines were studied by utilizing DNA microarrays containing over 21,000 human genes. The degree of respective lineage comitments were assessed by detection of lineage associated surface markers using flow cytometry. The rsults show the decrease in expression of HSC realted genes and proteins in G and Mk cultures. In Mk cells, the expression levels of granule proteins myeloperoxidase (MPO), cathepsin G (CTSG) and Charot-Leyden crystal protein (CLC) were comparable to or higher than those in G cells. G-cells displayed a high mRNA level of GATA-1, an important Mk transcription factor.

AB - The transcriptional programs of human hematopoietic stem cells (HSC) and early G and Mk differentiation grom human HSC in response to lineage-specific cytokines were studied by utilizing DNA microarrays containing over 21,000 human genes. The degree of respective lineage comitments were assessed by detection of lineage associated surface markers using flow cytometry. The rsults show the decrease in expression of HSC realted genes and proteins in G and Mk cultures. In Mk cells, the expression levels of granule proteins myeloperoxidase (MPO), cathepsin G (CTSG) and Charot-Leyden crystal protein (CLC) were comparable to or higher than those in G cells. G-cells displayed a high mRNA level of GATA-1, an important Mk transcription factor.

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M3 - Paper

AN - SCOPUS:22544464697

SP - 8341

T2 - 2004 AIChE Annual Meeting

Y2 - 7 November 2004 through 12 November 2004

ER -

Lineage plasticity and determinism in ex vivo differentiation of hematopoietic stem cells examined by large-scale transcriptional analysis

Abstract

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ASJC Scopus subject areas

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