Lineage plasticity and determinism in ex vivo differentiation of hematopoietic stem cells examined by large-scale transcriptional analysis

L. T. Huang*, C. Chen, E. T. Papoutsakis, William M Miller

*Corresponding author for this work

Research output: Contribution to conferencePaper

Abstract

The transcriptional programs of human hematopoietic stem cells (HSC) and early G and Mk differentiation grom human HSC in response to lineage-specific cytokines were studied by utilizing DNA microarrays containing over 21,000 human genes. The degree of respective lineage comitments were assessed by detection of lineage associated surface markers using flow cytometry. The rsults show the decrease in expression of HSC realted genes and proteins in G and Mk cultures. In Mk cells, the expression levels of granule proteins myeloperoxidase (MPO), cathepsin G (CTSG) and Charot-Leyden crystal protein (CLC) were comparable to or higher than those in G cells. G-cells displayed a high mRNA level of GATA-1, an important Mk transcription factor.

Original languageEnglish (US)
Number of pages1
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

ASJC Scopus subject areas

  • Engineering(all)

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    Huang, L. T., Chen, C., Papoutsakis, E. T., & Miller, W. M. (2004). Lineage plasticity and determinism in ex vivo differentiation of hematopoietic stem cells examined by large-scale transcriptional analysis. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.