Lipopeptides incorporated into supported phospholipid monolayers have high specific activity at low incorporation levels

Tor W. Jensen, Bi Huang Hu, Shara M. Delatore, Ana Sofia Garcia, Phillip B. Messersmith*, William M. Miller

*Corresponding author for this work

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The ability to present cell adhesion molecule (CAM) ligands in controlled amounts on a culture surface would greatly facilitate the control of cell growth and differentiation. Supported lipid monolayer/bilayer systems have previously been developed that allow for presentation of CAM ligands for cell interaction; however, these systems have employed peptide loadings much higher than those used in poly-(ethylene glycol) (PEG)-based immobilization systems. We report the development of synthetic methods that can be used for the efficient and versatile creation of many linear and cyclic lipid-linked peptide moieties. Using RGD-based peptides for the α5β1 integrin as a model system, we have demonstrated that these lipopeptides support efficient cell binding and spreading at CAM ligand loadings as low as 0.1 mol %, which is well below that previously reported for supported lipid systems. Engineered lipopeptide-based surfaces offer unique presentation options not possible with other immobilization systems, and the high activity at low loadings we have shown here may be extremely useful in presenting multiple CAM ligands for studying cell growth, differentiation, and signaling.

Original languageEnglish (US)
Pages (from-to)15223-15230
Number of pages8
JournalJournal of the American Chemical Society
Volume126
Issue number46
DOIs
StatePublished - Nov 24 2004

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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