Liposome-mediated herpes simplex virus uptake is glycoprotein-D receptor-independent but requires heparan sulfate

Lorrie A. Burnham, Dinesh Jaishankar, Jeffrey M. Thompson, Kevin S. Jones, Deepak Shukla, Vaibhav Tiwari*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Cationic liposomes are widely used to facilitate introduction of genetic material into target cells during transfection. This study describes a non-receptor mediated herpes simplex virus type-1 (HSV-1) entry into the Chinese hamster ovary (CHO-K1) cells that naturally lack glycoprotein D (gD)-receptors using a commercially available cationic liposome: Lipofectamine. Presence of cell surface heparan sulfate (HS) increased the levels of viral entry indicating a potential role of HS in this mode of entry. Loss of viral entry in the presence of actin de-polymerizing or lysosomotropic agents suggests that this mode of entry results in the endocytosis of the lipofectamine-virus mixture. Enhancement of HSV-1 entry by liposomes was also demonstrated in vivo using a zebrafish embryo model that showed stronger infection in the eyes and other tissues. Our study provides novel insights into gD receptor independent viral entry pathways and can guide new strategies to enhance the delivery of viral gene therapy vectors or oncolytic viruses.

Original languageEnglish (US)
Article number973
JournalFrontiers in Microbiology
Volume7
Issue numberJUN
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • Heparan sulfate
  • Viral entry
  • Virus-cell interactions

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Fingerprint

Dive into the research topics of 'Liposome-mediated herpes simplex virus uptake is glycoprotein-D receptor-independent but requires heparan sulfate'. Together they form a unique fingerprint.

Cite this