Live cell imaging of retroviral entry

Amy E. Hulme, Thomas J Hope

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Cellular entry of retroviruses is the first critical stage of retroviral replication. Live cell imaging has been utilized to visualize the dynamics, localization, and kinetics of the viral fusion process. Here, we review the different methodologies used for live cell imaging and how the use of these techniques has better elucidated the viral entry process of avian sarcoma and leukosis virus (ASLV) and human immunodeficiency virus type 1 (HIV-1) as well as cell-to-cell transmission of retroviruses. Although some controversies remain, further development of these techniques will provide new insights into the process and dynamics of retroviral fusion in vivo.

Original languageEnglish (US)
Pages (from-to)501-515
Number of pages15
JournalAnnual Review of Virology
Volume1
Issue number1
DOIs
StatePublished - Sep 29 2014

Keywords

  • ASLV
  • Cell-to-cell transmission
  • Fluorescence microscopy
  • HIV-1
  • Viral fusion

ASJC Scopus subject areas

  • Virology

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