Live cell microscopy of mitochondria-lysosome contact site formation and tethering dynamics

Tayler B. Belton, Eric D. Leisten, Jasmine Cisneros, Yvette C. Wong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Mitochondria-lysosome contact sites are critical for maintaining cellular homeostasis by regulating mitochondrial and lysosomal network dynamics and mediating metabolite exchange. Here, we present a protocol to quantitatively analyze the formation and tethering duration of mitochondria-lysosome contact sites by using time-lapse live confocal microscopy of LAMP1 and TOMM20. Although this protocol focuses on mammalian HeLa cells, it can be applied to other cell types for further studies on mitochondria-lysosome contact regulation and function, and elucidation of their role in human disorders. For complete details on the use and execution of this protocol, please refer to Wong et al. (2018) and Wong et al. (2019b).

Original languageEnglish (US)
Article number101262
JournalSTAR Protocols
Issue number2
StatePublished - Jun 17 2022


  • Cell Biology
  • Cell culture
  • Microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)


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