Lmo7 is dispensable for skeletal muscle and cardiac function

Dieu Hung Lao, Mary C. Esparza, Shannon N. Bremner, Indroneal Banerjee, Jianlin Zhang, Jennifer Veevers, William H. Bradford, Yusu Gu, Nancy D. Dalton, Kirk U. Knowlton, Kirk L. Peterson, Richard L. Lieber, Ju Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Emery-Dreifuss muscular dystrophy (EDMD) is a degenerative disease primarily affecting skeletal muscles in early childhood as well as cardiac muscle at later stages. EDMD is caused by a number of mutations in genes encoding proteins associated with the nuclear envelope (e.g., Emerin, Lamin A/C, and Nesprin). Recently, a novel protein, Lim-domain only 7 (lmo7) has been reported to play a role in the molecular pathogenesis of EDMD. Prior in vitro and in vivo studies suggested the intriguing possibility that Lmo7 plays a role in skeletal or cardiac muscle pathophysiology. To further understand the in vivo role of Lmo7 in striated muscles, we generated a novel Lmo7-null (lmo7−/−) mouse line. Using this mouse line, we examined skeletal and cardiac muscle physiology, as well as the role of Lmo7 in a model of muscular dystrophy and regeneration using the dystrophin-deficient mdxmouse model. Our results demonstrated that lmo7−/− mice had no abnormalities in skeletal muscle morphology, physiological function, or regeneration. Cardiac function was also unaffected. Moreover, we found that ablation of lmo7 in mdx mice had no effect on the observed myopathy and muscular regeneration exhibited by mdx mice. Molecular analyses also showed no changes in dystrophin complex factors, MAPK pathway components, and Emerin levels in lmo7 knockout mice. Taken together, we conclude that Lmo7 is dispensable for skeletal muscle and cardiac physiology and pathophysiology.

Original languageEnglish (US)
Pages (from-to)C470-C479
JournalAmerican Journal of Physiology - Cell Physiology
Volume309
Issue number7
DOIs
StatePublished - Sep 1 2015

Keywords

  • Cardiac muscle
  • Lim-domain only 7
  • Skeletal muscle
  • X-linked muscular dystrophy

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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