Local neurotrophin effects on central trigeminal axon growth patterns

P. Hande Ozdinler, Emel Ulupinar, Reha S. Erzurumlu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In dissociated cell and wholemount explant cultures of the embryonic trigeminal pathway NGF promotes exuberant elongation of trigeminal ganglion (TG) axons, whereas NT-3 leads to precocious arborization [J. Comp. Neurol. 425 (2000) 202]. In the present study, we investigated the axonal effects of local applications of NGF and NT-3. We placed small sepharose beads loaded with either NGF or NT-3 along the lateral edge of the central trigeminal tract in TG-brainstem intact wholemount explant cultures prepared from embryonic day 15 rats. Labeling of the TG with carbocyanine dye, DiI, revealed that NGF induces local defasciculation and diversion of trigeminal axons. Numerous axons leave the tract, grow towards the bead and engulf it, while some axons grow away from the neurotrophin source. NT-3, on the other hand, induced localized interstitial branching and formation of neuritic tangles in the vicinity of the neurotrophin source. Double immunocytochemistry showed that axons responding to NGF were predominantly TrkA-positive, whereas both TrkA and TrkC-positive axons responded to NT-3. Our results indicate that localized neurotrophin sources along the routes of embryonic sensory axons in the central nervous system, far away from their parent cell bodies, can alter restricted axonal pathways and induce elongation, arborization responses.

Original languageEnglish (US)
Pages (from-to)55-66
Number of pages12
JournalDevelopmental Brain Research
Volume151
Issue number1-2
DOIs
StatePublished - Jul 19 2004

Keywords

  • Axon arborization
  • Axon elongation
  • Development and regeneration
  • NGF
  • NT-3
  • Neurotrophic factors: biological effects
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Fingerprint

Dive into the research topics of 'Local neurotrophin effects on central trigeminal axon growth patterns'. Together they form a unique fingerprint.

Cite this