TY - JOUR
T1 - Local neurotrophin effects on central trigeminal axon growth patterns
AU - Ozdinler, P. Hande
AU - Ulupinar, Emel
AU - Erzurumlu, Reha S.
N1 - Funding Information:
Research supported by NIH/NIDCR. We thank Graham Balcer for his skillful assistance with several of the experiments, Louis Reichardt for Trk antibodies, and Ken Muneoka for sepharose beads. We are also grateful to Luis Marrero for his expert help with confocal imaging at the LSUHSC Morphology and Imaging Core.
PY - 2004/7/19
Y1 - 2004/7/19
N2 - In dissociated cell and wholemount explant cultures of the embryonic trigeminal pathway NGF promotes exuberant elongation of trigeminal ganglion (TG) axons, whereas NT-3 leads to precocious arborization [J. Comp. Neurol. 425 (2000) 202]. In the present study, we investigated the axonal effects of local applications of NGF and NT-3. We placed small sepharose beads loaded with either NGF or NT-3 along the lateral edge of the central trigeminal tract in TG-brainstem intact wholemount explant cultures prepared from embryonic day 15 rats. Labeling of the TG with carbocyanine dye, DiI, revealed that NGF induces local defasciculation and diversion of trigeminal axons. Numerous axons leave the tract, grow towards the bead and engulf it, while some axons grow away from the neurotrophin source. NT-3, on the other hand, induced localized interstitial branching and formation of neuritic tangles in the vicinity of the neurotrophin source. Double immunocytochemistry showed that axons responding to NGF were predominantly TrkA-positive, whereas both TrkA and TrkC-positive axons responded to NT-3. Our results indicate that localized neurotrophin sources along the routes of embryonic sensory axons in the central nervous system, far away from their parent cell bodies, can alter restricted axonal pathways and induce elongation, arborization responses.
AB - In dissociated cell and wholemount explant cultures of the embryonic trigeminal pathway NGF promotes exuberant elongation of trigeminal ganglion (TG) axons, whereas NT-3 leads to precocious arborization [J. Comp. Neurol. 425 (2000) 202]. In the present study, we investigated the axonal effects of local applications of NGF and NT-3. We placed small sepharose beads loaded with either NGF or NT-3 along the lateral edge of the central trigeminal tract in TG-brainstem intact wholemount explant cultures prepared from embryonic day 15 rats. Labeling of the TG with carbocyanine dye, DiI, revealed that NGF induces local defasciculation and diversion of trigeminal axons. Numerous axons leave the tract, grow towards the bead and engulf it, while some axons grow away from the neurotrophin source. NT-3, on the other hand, induced localized interstitial branching and formation of neuritic tangles in the vicinity of the neurotrophin source. Double immunocytochemistry showed that axons responding to NGF were predominantly TrkA-positive, whereas both TrkA and TrkC-positive axons responded to NT-3. Our results indicate that localized neurotrophin sources along the routes of embryonic sensory axons in the central nervous system, far away from their parent cell bodies, can alter restricted axonal pathways and induce elongation, arborization responses.
KW - Axon arborization
KW - Axon elongation
KW - Development and regeneration
KW - NGF
KW - NT-3
KW - Neurotrophic factors: biological effects
KW - Trigeminal ganglion
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U2 - 10.1016/j.devbrainres.2004.03.017
DO - 10.1016/j.devbrainres.2004.03.017
M3 - Article
C2 - 15246692
AN - SCOPUS:3042853070
VL - 151
SP - 55
EP - 66
JO - Developmental Brain Research
JF - Developmental Brain Research
SN - 0165-3806
IS - 1-2
ER -