Local production of the chemokines CCL5 and CXCL10 attracts CD8++ T lymphocytes into esophageal squamous cell carcinoma

Jinyan Liu, Feng Li, Yu Ping, Liping Wang, Xinfeng Chen, Dan Wang, Ling Cao, Song Zhao, Bing Li, Pawel Kalinski, Stephen H. Thorne, Bin Zhang, Yi Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Esophageal squamous cell carcinoma (ESCC) is a very common malignant tumor with poor prognosis in China. Chemokines secreted by tumors are pivotal for the accumulation of CD8++ T lymphocytes within malignant lesions in several types of cancers, but the exact mechanism underlying CD8++ T lymphocyte homing is still unknown in ESCC. In this study, we revealed that, compared with marginal tissues, the expression of both chemokine (C-C motif) ligand 5 (CCL5) and (C-X-C motif) ligand 10 (CXCL10) was upregulated in ESCC tissues. CCL5 expression was positively associated with the overall survival of patients. Meanwhile, RT-PCR data showed that the expression of CCL5 and CXCL10 was positively correlated with the local expressions of the CD8++ T lymphocyte markers (CD8+ and Granzyme B) in tumor tissues. Correspondingly, CD8++ T lymphocytes were more frequently CCR5- and CXCR3- positive in tumor than in peripheral blood. Transwell analysis showed both CCL5 and CXCL10 were important for the chemotactic movement of CD8++ T lymphocytes. Our data indicate that CCL5 and CXCL10 serve as the key chemokines to recruit CD8++ T lymphocytes into ESCC tissue and may play a role in patient survival.

Original languageEnglish (US)
Pages (from-to)24978-24989
Number of pages12
JournalOncotarget
Volume6
Issue number28
DOIs
StatePublished - 2015

Keywords

  • CCL5
  • CXCL10
  • Chemotaxis
  • Esophageal squamous cell carcinoma
  • T lymphocyte

ASJC Scopus subject areas

  • Oncology

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