Localization of MAP kinase activity in early Xenopus embryos: Implications for endogenous FGF signaling

C. LaBonne; M. Whitman

doi:10.1006/dbio.1996.8497

Localization of MAP kinase activity in early Xenopus embryos: Implications for endogenous FGF signaling

C. LaBonne, M. Whitman^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

We have used a sensitive assay for MAP kinase activity to investigate the role of endogenous fibroblast growth factor (FGF)-activated MAP kinase in early Xenopus embryonic patterning. MAP kinase activity is low during cleavage stages and increases significantly during gastrulation. The temporal profile of this activity correlates well with the expression pattern of Xenopus eFGF. Spatially, MAP kinase activity is lowest in animal pole tissue and higher in vegetal pole cells and the marginal zone. Endogenous MAP kinase activity is FGF receptor-dependent, demonstrating that FGF signaling is active in all three germ layers of the early embryo. This activity is necessary for normal expression of Mix.1, a mesoendodermal marker, in the endoderm as well as in the mesoderm, indicating that MAP kinase plays a functional role in patterning of both of these germ layers. Spatial and temporal changes in MAP kinase activation during gastrulation also suggest a role for FGF signaling in this process. In addition, we find that embryonic wounding during dissection results in significant stimulation of this pathway, providing a possible explanation for earlier observations of effects of surgical manipulation on cell fate in early embryos.

Original language	English (US)
Pages (from-to)	9-20
Number of pages	12
Journal	Developmental Biology
Volume	183
Issue number	1
DOIs	https://doi.org/10.1006/dbio.1996.8497
State	Published - Mar 1 1997

Funding

The authors gratefully acknowledge Jonkyeung Chung and John Blenis for providing STAT-3 substrate and sharing results prior to publication and Rebecca Hartley and Jim Maller for MAP kinase speci®c antibodies. We thank Anna Philpott, Jeremy Green, Karen Symes, and Adrian Gross for helpful discussions. We are grateful to Karen Symes, Jeremy Green, and Ellen Weisberg for critical reading of the manuscript. C.L. was supported, in part, by an NSF predoctoral fellowship. M.W. is a scholar of the Lucille P. Markey Charitable Trust. This work was also supported by a grant from the NIH.

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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title = "Localization of MAP kinase activity in early Xenopus embryos: Implications for endogenous FGF signaling",

abstract = "We have used a sensitive assay for MAP kinase activity to investigate the role of endogenous fibroblast growth factor (FGF)-activated MAP kinase in early Xenopus embryonic patterning. MAP kinase activity is low during cleavage stages and increases significantly during gastrulation. The temporal profile of this activity correlates well with the expression pattern of Xenopus eFGF. Spatially, MAP kinase activity is lowest in animal pole tissue and higher in vegetal pole cells and the marginal zone. Endogenous MAP kinase activity is FGF receptor-dependent, demonstrating that FGF signaling is active in all three germ layers of the early embryo. This activity is necessary for normal expression of Mix.1, a mesoendodermal marker, in the endoderm as well as in the mesoderm, indicating that MAP kinase plays a functional role in patterning of both of these germ layers. Spatial and temporal changes in MAP kinase activation during gastrulation also suggest a role for FGF signaling in this process. In addition, we find that embryonic wounding during dissection results in significant stimulation of this pathway, providing a possible explanation for earlier observations of effects of surgical manipulation on cell fate in early embryos.",

author = "C. LaBonne and M. Whitman",

note = "Funding Information: The authors gratefully acknowledge Jonkyeung Chung and John Blenis for providing STAT-3 substrate and sharing results prior to publication and Rebecca Hartley and Jim Maller for MAP kinase speci{\textregistered}c antibodies. We thank Anna Philpott, Jeremy Green, Karen Symes, and Adrian Gross for helpful discussions. We are grateful to Karen Symes, Jeremy Green, and Ellen Weisberg for critical reading of the manuscript. C.L. was supported, in part, by an NSF predoctoral fellowship. M.W. is a scholar of the Lucille P. Markey Charitable Trust. This work was also supported by a grant from the NIH.",

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N2 - We have used a sensitive assay for MAP kinase activity to investigate the role of endogenous fibroblast growth factor (FGF)-activated MAP kinase in early Xenopus embryonic patterning. MAP kinase activity is low during cleavage stages and increases significantly during gastrulation. The temporal profile of this activity correlates well with the expression pattern of Xenopus eFGF. Spatially, MAP kinase activity is lowest in animal pole tissue and higher in vegetal pole cells and the marginal zone. Endogenous MAP kinase activity is FGF receptor-dependent, demonstrating that FGF signaling is active in all three germ layers of the early embryo. This activity is necessary for normal expression of Mix.1, a mesoendodermal marker, in the endoderm as well as in the mesoderm, indicating that MAP kinase plays a functional role in patterning of both of these germ layers. Spatial and temporal changes in MAP kinase activation during gastrulation also suggest a role for FGF signaling in this process. In addition, we find that embryonic wounding during dissection results in significant stimulation of this pathway, providing a possible explanation for earlier observations of effects of surgical manipulation on cell fate in early embryos.

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Localization of MAP kinase activity in early Xenopus embryos: Implications for endogenous FGF signaling

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