Localization of peroxisome proliferator-activated receptor in mouse and rat tissues and demonstration of its nuclear translocation in transfected CV-1 cells

Q. Huang*, Anjana V Yeldandi, Keith Alvares, H. Ide, Janardan K Reddy, Sambasiva Rao Musunuri

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Hepatocarcinogenesis in rodents induced by nongenotoxic peroxisome proliferators is postulated to be a receptor-mediated process. The peroxisome proliferator-activated receptors (PPAR) are members of the steroid hormone receptor superfamily, which participate in ligand-dependent transcriptional activation of peroxisomal fatty acid β oxidation enzyme system genes in liver parenchymal cells of rats and mice. In order to study the tissue distribution and cellular localization of PPAR, we raised polyclonal antibodies against PPAR using a recombinant rat PPAR (rPPAR) expressed as a glutathione-S-transferase-rPPAR fusion protein. On immunoblot analysis the antibodies specifically recognized a 55 kDa PPAR protein in rat, mouse and human liver homogenates. Immunoblotting also showed that in the mouse and rat, PPAR is expressed in liver, kidney and heart, and only weakly in brain and testis. Immunohistochemical localization in the rat and mouse revealed that PPAR is highly expressed in perivenular (i.e., those surrounding hepatic vein) hepatocytes and very weakly in the cytoplasm of remaining hepatocytes. In the kidney, PPAR was visualized predominantly in the p3 segments of proximal convoluted tubular epithelium. CV-1 cells transiently transfected with rPPAR cDNA construct showed predominant cytoplasmic fluorescence; treatment of these cells with ciprofibrate, a peroxisome proliferator, resulted in the nuclear translocation of PPAR signal.

Original languageEnglish (US)
Pages (from-to)307-312
Number of pages6
JournalInternational Journal of Oncology
Volume6
Issue number2
StatePublished - Jan 1 1995

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Keywords

  • Nuclear translocation
  • Peroxisome proliferator-activated receptor
  • Peroxisome proliferators
  • Peroxisomes
  • Polyclonal antibodies

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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