Localization of the alpha 3 (V) chain of type V collagen in human skin

David T. Woodley*, Vicki J. Scheidt, Melinda J. Reese, Amy S. Paller, Thomas O. Manning, Takashi Yoshiike, Robert A. Briggaman

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Serum from goats immunized with human type V collagen chains that were cut out of polyacrylamide gels contained an antibody that recognized only type V collagen in an enzyme-linked immunoabsorbent assay and did not label laminin, fibronectin, or types I and IV collagen. Western blot analysis of the antibody showed that its determinant was the alpha 3 (V) chain of type V collagen. Indirect immunofluorescent staining of intact human skin with the antibody produced staining of the dermal blood vessels but not of the dermal-epidermal junction (DEJ). In contrast, both the dermal blood vessels and the DEJ were labeled by the antibody if the skin substrate was first split through the lamina lucida region of the DEJ by incubation in 1 M NaCl solution. Indirect immunoelectron microscopy confirmed the staining pattern found by immunofluorescence and defined the ultrastructural localization of type V collagen in skin. Type V collagen is localized within the DEJ to the lamina lucida region and polar aspects of the basal cell keratinocyte plasma membrane.

Original languageEnglish (US)
Pages (from-to)246-252
Number of pages7
JournalJournal of Investigative Dermatology
Volume88
Issue number3
DOIs
StatePublished - Mar 1987

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Fingerprint Dive into the research topics of 'Localization of the alpha 3 (V) chain of type V collagen in human skin'. Together they form a unique fingerprint.

  • Cite this

    Woodley, D. T., Scheidt, V. J., Reese, M. J., Paller, A. S., Manning, T. O., Yoshiike, T., & Briggaman, R. A. (1987). Localization of the alpha 3 (V) chain of type V collagen in human skin. Journal of Investigative Dermatology, 88(3), 246-252. https://doi.org/10.1111/1523-1747.ep12465467