Locomotion of monocytes on endothelium is a critical step during extravasation

Alan R. Schenkel; Zahra Mamdouh; William A. Muller

doi:10.1038/ni1051

Locomotion of monocytes on endothelium is a critical step during extravasation

Alan R. Schenkel^*, Zahra Mamdouh, William A. Muller

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

356 Scopus citations

Abstract

Monocytes, like all leukocytes, undergo a series of sequential steps during extravasation from blood into tissues: tethering, rolling, adhesion and diapedesis. We have discovered an essential step, which we call locomotion, in which the monocyte moves from a site of firm adhesion to the nearest junction to begin diapedesis. Blocking CD11a-CD18 and CD11b-CD18 on human monocytes or adhesion molecules ICAM-1 and ICAM-2 on endothelial cells prevented the monocytes from reaching junctions. The blocked monocytes spun in circles as if they were unable to direct their movement despite being able to adhere and polarize normally. This step fills a gap in the paradigm of extravasation as a multistep process.

Original language	English (US)
Pages (from-to)	393-400
Number of pages	8
Journal	Nature Immunology
Volume	5
Issue number	4
DOIs	https://doi.org/10.1038/ni1051
State	Published - Apr 2004

Funding

We acknowledge R. Liebman for technical support and tissue culture, and L. Pierini for critical review of this manuscript. Supported by National Institutes of Health HL46849 (W.A.M.), HL64774 (W.A.M.), and HL10311 (A.R.S.) and in part by the Charles H. Revson Foundation (A.R.S. and Z.M.).

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1038/ni1051

Cite this

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title = "Locomotion of monocytes on endothelium is a critical step during extravasation",

abstract = "Monocytes, like all leukocytes, undergo a series of sequential steps during extravasation from blood into tissues: tethering, rolling, adhesion and diapedesis. We have discovered an essential step, which we call locomotion, in which the monocyte moves from a site of firm adhesion to the nearest junction to begin diapedesis. Blocking CD11a-CD18 and CD11b-CD18 on human monocytes or adhesion molecules ICAM-1 and ICAM-2 on endothelial cells prevented the monocytes from reaching junctions. The blocked monocytes spun in circles as if they were unable to direct their movement despite being able to adhere and polarize normally. This step fills a gap in the paradigm of extravasation as a multistep process.",

author = "Schenkel, {Alan R.} and Zahra Mamdouh and Muller, {William A.}",

note = "Funding Information: We acknowledge R. Liebman for technical support and tissue culture, and L. Pierini for critical review of this manuscript. Supported by National Institutes of Health HL46849 (W.A.M.), HL64774 (W.A.M.), and HL10311 (A.R.S.) and in part by the Charles H. Revson Foundation (A.R.S. and Z.M.).",

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AU - Schenkel, Alan R.

AU - Mamdouh, Zahra

AU - Muller, William A.

N1 - Funding Information: We acknowledge R. Liebman for technical support and tissue culture, and L. Pierini for critical review of this manuscript. Supported by National Institutes of Health HL46849 (W.A.M.), HL64774 (W.A.M.), and HL10311 (A.R.S.) and in part by the Charles H. Revson Foundation (A.R.S. and Z.M.).

PY - 2004/4

Y1 - 2004/4

N2 - Monocytes, like all leukocytes, undergo a series of sequential steps during extravasation from blood into tissues: tethering, rolling, adhesion and diapedesis. We have discovered an essential step, which we call locomotion, in which the monocyte moves from a site of firm adhesion to the nearest junction to begin diapedesis. Blocking CD11a-CD18 and CD11b-CD18 on human monocytes or adhesion molecules ICAM-1 and ICAM-2 on endothelial cells prevented the monocytes from reaching junctions. The blocked monocytes spun in circles as if they were unable to direct their movement despite being able to adhere and polarize normally. This step fills a gap in the paradigm of extravasation as a multistep process.

AB - Monocytes, like all leukocytes, undergo a series of sequential steps during extravasation from blood into tissues: tethering, rolling, adhesion and diapedesis. We have discovered an essential step, which we call locomotion, in which the monocyte moves from a site of firm adhesion to the nearest junction to begin diapedesis. Blocking CD11a-CD18 and CD11b-CD18 on human monocytes or adhesion molecules ICAM-1 and ICAM-2 on endothelial cells prevented the monocytes from reaching junctions. The blocked monocytes spun in circles as if they were unable to direct their movement despite being able to adhere and polarize normally. This step fills a gap in the paradigm of extravasation as a multistep process.

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Locomotion of monocytes on endothelium is a critical step during extravasation

Abstract

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ASJC Scopus subject areas

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