Abstract
The hierarchical relationships between various stem and progenitor cell subpopulations driving mammary gland morphogenesis and homoeostasis are poorly understood. Conditional inactivation experiments previously demonstrated that expression of the zinc finger transcriptional repressor Blimp1/PRDM1 is essential for the establishment of epithelial cell polarity and functional maturation of alveolar cells. Here we exploit a Prdm1.CreERT2-LacZ reporter allele for lineage tracing experiments. Blimp1 expression marks a rare subpopulation of unipotent luminal stem cells that initially appear in the embryonic mammary gland at around E17.5 coincident with the segregation of the luminal and basal compartments. Fate mapping at multiple time points in combination with whole-mount confocal imaging revealed these long-lived unipotent luminal stem cells survive consecutive involutions and retain their identity throughout adult life. Blimp1+ luminal stem cells give rise to Blimp1- progeny that are invariably Elf5+ERα-PR-. Thus, Blimp1 expression defines a mammary stem cell subpopulation with unique functional characteristics.
| Original language | English (US) |
|---|---|
| Article number | 1714 |
| Journal | Nature communications |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1 2017 |
Funding
We thank Michal Maj and Alan Wainman for valuable assistance with flow cytometry and confocal microscopy, respectively; Jane Rose and Thomas Clague for mouse husbandry; and Xin Lu (University of Oxford) for providing the K14-Cre mouse strain. This work was funded by a grant from the Wellcome Trust [WT 102811]. E.J.R. is a Wellcome Trust Principal Research Fellow.
ASJC Scopus subject areas
- General Physics and Astronomy
- General Chemistry
- General Biochemistry, Genetics and Molecular Biology