Longitudinal deep-brain imaging in mouse using visible-light optical coherence tomography through chronic microprism cranial window

Lisa Beckmann, Xian Zhang, Neil A. Nadkarni, Zhen Cai, Ayush Batra, David P. Sullivan, William A. Muller, Cheng Sun, Roman Kuranov, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We longitudinally imaged both the superficial and deep cortical microvascular networks in brains of healthy mice and in a mouse model of stroke in vivo using visible-light optical coherence tomography (vis-OCT). We surgically implanted a microprism in mouse brains sealed by a chronic cranial window. The microprism enabled vis-OCT to image the entire depth of the mouse cortex. Following microprism implantation, we imaged the mice for 28 days and found that that it took around 15 days for both the superficial and deep cortical microvessels to recover from the implantation surgery. After the brains recovered, we introduced ischemic strokes by transient middle cerebral artery occlusion (tMCAO). We monitored the strokes for up to 60 days and observed different microvascular responses to tMCAO at different cortical depths in both the acute and chronic phases of the stroke. This work demonstrates that the combined microprism and cranial window is well-suited for longitudinal investigation of cortical microvascular disorders using vis-OCT.

Original languageEnglish (US)
Pages (from-to)5235-5250
Number of pages16
JournalBiomedical Optics Express
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2019

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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