Abstract
Hyperreflective foci (HRFs) appear in optical coherence tomography (OCT) images of the retina and vitreous of patients with various ocular diseases. HRFs are hypothesized to be immune cells that appear in response to ischemia or tissue damage. To accurately identify HRFs and establish their clinical significance, it is necessary to replicate the detection of similar patterns in vivo in a small animal model. We combined visible-light OCT with temporal speckle averaging (TSA) to visualize and track vitreal HRFs (VHRFs) densities for three days after an optic nerve crush (ONC) injury. Resulting vis-OCT images revealed that VHRF density significantly increased approximately 10-fold at 12 h after ONC and returned to baseline three days after ONC. Additional immunohistochemistry results confirmed these VHRFs as inflammatory cells induced from optic nerve damage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1880-1883 |
| Number of pages | 4 |
| Journal | Optics Letters |
| Volume | 49 |
| Issue number | 8 |
| DOIs | |
| State | Published - Apr 15 2024 |
Funding
Funding. National Institutes of Health (R01EY029121, R01EY033813, R01EY034353, R01EY034740, R44EY026466, U01EY033001); Christine Enroth-Cugell and David Cugell Fellowship for Visual Neuroscience and Biomedical Engineering.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics