Lrp5/b-Catenin signaling controls lung macrophage differentiation and inhibits resolution of fibrosis

Joseph A. Sennello, Alexander V. Misharin, Annette S. Flozak, Sergejs Berdnikovs, Paul Cheresh, John Varga, David W. Kamp, G. R.Scott Budinger, Cara J. Gottardi, Anna P. Lam*

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Previous studies established that attenuatingWnt/β-catenin signaling limits lung fibrosis in the bleomycinmouse model of this disease, but the contribution of this pathway to distinct lung cell phenotypes relevant to tissue repair and fibrosis remains incompletely understood. Using microarray analysis, we found that bleomycin-injured lungs from mice that lack theWnt coreceptor low density lipoprotein receptor-related protein 5 (Lrp5) and exhibit reduced fibrosis showed enrichment for pathways related to extracellular matrix processing, immunity, and lymphocyte proliferation, suggesting the contribution of an immunematrix remodeling axis relevant to fibrosis. Activation of β-catenin signaling was seen in lung macrophages using the β-catenin reporter mouse, Axin2+/LacZ. Analysis of lung immune cells by flow cytometry after bleomycin administration revealed that Lrp5-/-lungs contained significantly fewer Siglec Flowalveolar macrophages, a cell type previously implicated as positive effectors of fibrosis. Macrophagespecific deletion of β-catenin in CD11ccre;β-cateninflox mice did not prevent development of bleomycin-induced fibrosis but facilitated its resolution by 8 weeks. In a nonresolvingmodel of fibrosis, intratracheal administration of asbestos in Lrp5-/- mice also did not prevent the development of fibrosis but hindered the progression of fibrosis in asbestos-Treated Lrp5-/- lungs, phenocopying the findings in bleomycin-Treated CD11ccre;β-cateninflox mice. Activation of β-catenin signaling using lithium chloride resulted in worsened fibrosis in wild-Type mice, further supporting that the effects of loss of Lrp5 are directly mediated by Wnt/β-catenin signaling. Together, these data suggest that lung myeloid cells are responsive to Lrp5/β-catenin signaling, leading to differentiation of an alveolar macrophage subtype that antagonizes the resolution of lung fibrosis.

Original languageEnglish (US)
Pages (from-to)191-201
Number of pages11
JournalAmerican journal of respiratory cell and molecular biology
Volume56
Issue number2
DOIs
StatePublished - Feb 2017

Keywords

  • Alveolar macrophage
  • Bleomycin
  • Lrp5
  • Lung fibrosis
  • β-catenin

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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