LSPR biosensor signal enhancement using nanoparticle-antibody conjugates

W. Paige Hall, Salome N. Ngatia, Richard P. Van Duyne

Research output: Contribution to journalArticlepeer-review

212 Scopus citations


A method to amplify the wavelength shift observed from localized surface plasmon resonance (LSPR) bioassays is developed using gold nanoparticle-labeled antibodies. The technique, which involves detecting surface-bound analytes using gold nanoparticle conjugated antibodies, provides a way to enhance LSPR shifts for more sensitive detection of low-concentration analytes. Using the biotin and antibiotin binding pair as a model, we demonstrate up to a 400% amplification of the shift upon antibody binding to analyte. In addition, the antibody-nanoparticle conjugate improves the observed binding constant by 2 orders of magnitude, and the limit of detection by nearly 3 orders of magnitude. This amplification strategy provides a way to improve the sensitivity of plasmon-based bioassays, paving the way for single molecule-based detection and clinically relevant diagnostics.

Original languageEnglish (US)
Pages (from-to)1410-1414
Number of pages5
JournalJournal of Physical Chemistry C
Issue number5
StatePublished - Feb 10 2011

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • General Energy
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films


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