Lung angiotensin-converting enzyme kinetics from indicator-dilution and constant-infusion methods

J. H. Linehan*, R. D. Bongard, D. L. Roerig, T. A. Bronikowski, C. A. Dawson

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We compared the results of three methods used to evaluate the kinetics of benzoyl-phenylalanyl-alanyl-proline (BPAP) hydrolysis by angiotensin-converting enzyme in the isolated rabbit lung. In method A, the arteriovenous concentration differences at each of four rates of BPAP infusion were used to determine the effect of concentration on the rate of BPAP hydrolysis. In methods B1 and B2, trace doses of [3H]BPAP were injected during each constant infusion of unlabeled BPAP so that the venous [3H]BPAP concentration could be used to estimate the venous BPAP concentration and the rate of hydrolysis at each background concentration. In method C, three boluses containing different amounts of unlabeled BPAP as well as tracer [3H]BPAP were injected such that each bolus resulted in a range of concentrations and hydrolysis rates that could be estimated from the venous concentrations of [3H]BPAP. Each method provided the data needed to calculate the maximum uptake rate (V(max)) and K(m), the concentration at half V(max), assuming that the hydrolysis can be represented by the Michaelis-Menten equation. However, the mathematical model underlying each method involved different assumptions about the effects of heterogeneity of capillary transit times and bolus dispersion. The mean values of V(max) were 180, 216, 217, and 200 nmol/s and for K(m) 7.8, 10.4, 8.6, and 10.0 μM for methods A, B1, B2, and C, respectively. The differences between methods were not statistically significant. These results suggest that theoretical differences between the methods did not have a quantitatively important impact relative to other factors contributing random errors to each of the methods. The choice between methods can therefore be made on practical grounds.

Original languageEnglish (US)
Pages (from-to)H436-H444
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume258
Issue number2 27-2
DOIs
StatePublished - 1990

Keywords

  • Michaelis-Menten equation
  • high-performance liquid chromatography
  • isolated rabbit lung
  • pulmonary endothelium
  • radiolabeled tracer

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Lung angiotensin-converting enzyme kinetics from indicator-dilution and constant-infusion methods'. Together they form a unique fingerprint.

  • Cite this