Lung liquid clearance and NA,K-ATPase during acute hyperoxia and recovery in rats

W. G. Olivera, K. M. Ridge, J. I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


Lung liquid clearance, epithelial permeability for Na+, mannitol and albumin, as well as Na,K-ATPase activity in alveolar type 2 (AT2) cells were studied during the acute and the recovery phase of hyperoxic lung injury. Rats exposed to 100% oxygen for 64 h were studied at 0, 7 and 14 d after removal from the hyperoxic chamber and compared with control rats breathing room air. In the isolated-perfused, liquid-filled rat lung, the albumin flux from the perfusate into the air spaces increased immediately after the oxygen exposure (220 ± 56 mg/h) and returned to control values (28 ± 7 mg/h) after 7 and 14 d of recovery. The small solutes (Na+ and mannitol) flux across the alveolar epithelium normalized only after 14 d of recovery in room air. Active Na+ transport and lung liquid clearance were reduced by 445% immediately after oxygen exposure when compared with control values, increased by ~56% above control values after 7 d of recovery, and returned to control values after 14 d of recovery. Paralleling these changes the Na, K-ATPase activity decreased by ~41% in AT2 cells isolated from rats after 64 h of breathing 100% O2 and increased by ~25% after the rats recovered in room air for 7 d. These results suggest that alveolar epithelial Na,K-ATPase may contribute in the recovery from the hyperoxic lung injury by participating in the clearance of lung edema.

Original languageEnglish (US)
Pages (from-to)1229-1234
Number of pages6
JournalAmerican journal of respiratory and critical care medicine
Issue number4 I
StatePublished - Oct 1995

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


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