Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon

Jordan S. Pober*, Tucker Collins, Michael A. Gimbrone, Ramzi S. Cotran, Jonathan D. Gitlin, Walter Fiers, Carol Clayberger, Alan M. Krensky, Steven J. Burakoff, Carol S. Reiss

*Corresponding author for this work

Research output: Contribution to journalArticle

368 Scopus citations

Abstract

T-lymphocyte-mediated responses to the cellular components of blood vessels are important in rejection of allografts1-3. The induction of cytolytic T lymphocytes (CTLs) depends on recognition of foreign class II major histocompatibility complex antigens (human HLA-DR, DC/DS, SB and others, collectively referred to as Ia) on the target cells whereas killing by CTLs usually depends on recognition of foreign class I antigens (HLA-A,B) 4, although some alloreactive CTLs recognize foreign Ia instead of HLA-A, B (refs 5-8). The expression of Ia antigens has traditionally been regarded as restricted to immunological cell types, and the presence of class II antigen-bearing 'passenger' leukocytes in rodent organ grafts appears necessary for graft rejection9-11. Recently, Ia antigens have been observed by immunofluorescence microscopy on human renal and dermal capillary endothelium12-15. We have previously shown that human umbilical vein endothelial (HUVE) cells in standard culture conditions do not bear Ia antigens, but may be induced to do so by products of lectin- or alloantigen-activated T lymphocytes16,17. Furthermore, we found that recombinant immune interferon (IFN-γ), free of other lymphokines, is a potent inducer of Ia expression in HUVE cells17. Here we report that IFN-γ also induces Ia expression on human foreskin capillary endothelial (HFCE) cells, HUVE cells transformed by Simian virus 40 viral DNA (SV-HUVE cells) and human dermal fibroblast (HDF) cells in culture. Further, we present evidence that Ia present on HUVE cells and HDF cells can be functionally recognized by human T cells, resulting in a two-way interaction between T cells and mesenchymal cells that may be important in allograft rejection.

Original languageEnglish (US)
Pages (from-to)726-729
Number of pages4
JournalNature
Volume305
Issue number5936
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • General

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    Pober, J. S., Collins, T., Gimbrone, M. A., Cotran, R. S., Gitlin, J. D., Fiers, W., Clayberger, C., Krensky, A. M., Burakoff, S. J., & Reiss, C. S. (1983). Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. Nature, 305(5936), 726-729. https://doi.org/10.1038/305726a0