Macro-scale topology optimization for controlling internal shear stress in a porous scaffold bioreactor

K. Youssef, J. J. Mack, M. L. Iruela-Arispe, L. S. Bouchard*

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Shear stress is an important physical factor that regulates proliferation, migration, and morphogenesis. In particular, the homeostasis of blood vessels is dependent on shear stress. To mimic this process ex vivo, efforts have been made to seed scaffolds with vascular and other cell types in the presence of growth factors and under pulsatile flow conditions. However, the resulting bioreactors lack information on shear stress and flow distributions within the scaffold. Consequently, it is difficult to interpret the effects of shear stress on cell function. Such knowledge would enable researchers to improve upon cell culture protocols. Recent work has focused on optimizing the microstructural parameters of the scaffold to fine tune the shear stress. In this study, we have adopted a different approach whereby flows are redirected throughout the bioreactor along channels patterned in the porous scaffold to yield shear stress distributions that are optimized for uniformity centered on a target value. A topology optimization algorithm coupled to computational fluid dynamics simulations was devised to this end. The channel topology in the porous scaffold was varied using a combination of genetic algorithm and fuzzy logic. The method is validated by experiments using magnetic resonance imaging readouts of the flow field.

Original languageEnglish (US)
Pages (from-to)1844-1854
Number of pages11
JournalBiotechnology and Bioengineering
Volume109
Issue number7
DOIs
StatePublished - Jul 1 2012

Keywords

  • Flow mapping
  • Flow simulation
  • Porous scaffold
  • Topology optimization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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