Macrophage Inflammatory Protein-Iβ: A C-C Chemokine in Osteoarthritis

Alisa E. Koch, Steven L. Kunkel, Manisha R. Shah, Rebekah Fu, Daphne D. Mazarakis, G. Kenneth Haines, Marie D. Burdick, Richard M. Pope, Robert M. Strieter

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

The aim of this study was to determine whether the cytokine macrophage inflammatory protein-1β (MIP1β) is present and functionally active in the arthritic joint. We used immunoassays and bioassays to assess the presence and function of MIP-1β using samples obtained from 62 arthritic patients. MIP-1β levels were increased in synovial fluids (SFs) from patients with osteoarthritis (OA) (18.0 ± 8.9 ng/ml) (SD) compared to patients with rheumatoid arthritis (RA) (6.1 ± 2.9 ng/ml) or other forms of arthritis (10.4 ± 7.0 ng/ml) (P < 0.05). Levels of OA SF MIP-1β were significantly greater than OA or normal serum levels of MIP-1β. Anti-MIP-1β neutralized 28% of the chemotactic activity for monocytes found in OA SFs. Isolated OA synovial tissue fibroblasts did not constitutively produce MIP-1β but could be induced to express this chemokine upon exposure to tumor necrosis factor-α, interleukin-1β, or lipopolysaccharide. Synovial tissue immunohistochemical staining revealed that the main immunopositive cells in OA were the lining cells as well as vascular smooth muscle and endothelial cells. A minority of macrophages were immunopositive as well. In this study, we identify MIP-1β as a unique cytokine increased in OA compared to RA SF. We conclude that MIP-1β may play a role in the ingress of monocytes into the OA joint.

Original languageEnglish (US)
Pages (from-to)307-314
Number of pages8
JournalClinical Immunology and Immunopathology
Volume77
Issue number3
DOIs
StatePublished - Dec 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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