Macrophage tropism determinants of human immunodeficiency virus type 1 in vivo

P. Westervelt; D. B. Trowbridge; L. G. Epstein; B. M. Blumberg; Y. Li; B. H. Hahn; G. M. Shaw; R. W. Price; L. Ratner

Macrophage tropism determinants of human immunodeficiency virus type 1 in vivo

P. Westervelt, D. B. Trowbridge, L. G. Epstein, B. M. Blumberg, Y. Li, B. H. Hahn, G. M. Shaw, R. W. Price, L. Ratner^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

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Abstract

Strains of human immunodeficiency virus type 1 differ in their abilities to infect and replicate in primary human macrophages. Chimeric clones were constructed from a provirus unable to infect macrophages (NLHX) and envelope sequences (V3 loop) of viruses derived without cultivation from brain (YU2 and w1-1c1) or spleen (w2-1b4) tissues. The substituted V3 loop sequences in each case were sufficient to confer upon NLHX the ability to infect macrophages. Furthermore, an envelope domain immediately N terminal to the V3 loop also was found to modulate the level of replication in macrophages. These results demonstrate that an envelope determinant derived directly from patients with AIDS confers HFV-1 tropism for macrophages.

Original language	English (US)
Pages (from-to)	2577-2582
Number of pages	6
Journal	Journal of virology
Volume	66
Issue number	4
State	Published - Apr 1992

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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title = "Macrophage tropism determinants of human immunodeficiency virus type 1 in vivo",

abstract = "Strains of human immunodeficiency virus type 1 differ in their abilities to infect and replicate in primary human macrophages. Chimeric clones were constructed from a provirus unable to infect macrophages (NLHX) and envelope sequences (V3 loop) of viruses derived without cultivation from brain (YU2 and w1-1c1) or spleen (w2-1b4) tissues. The substituted V3 loop sequences in each case were sufficient to confer upon NLHX the ability to infect macrophages. Furthermore, an envelope domain immediately N terminal to the V3 loop also was found to modulate the level of replication in macrophages. These results demonstrate that an envelope determinant derived directly from patients with AIDS confers HFV-1 tropism for macrophages.",

author = "P. Westervelt and Trowbridge, {D. B.} and Epstein, {L. G.} and Blumberg, {B. M.} and Y. Li and Hahn, {B. H.} and Shaw, {G. M.} and Price, {R. W.} and L. Ratner",

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language = "English (US)",

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T1 - Macrophage tropism determinants of human immunodeficiency virus type 1 in vivo

AU - Westervelt, P.

AU - Trowbridge, D. B.

AU - Epstein, L. G.

AU - Blumberg, B. M.

AU - Li, Y.

AU - Hahn, B. H.

AU - Shaw, G. M.

AU - Price, R. W.

AU - Ratner, L.

PY - 1992/4

Y1 - 1992/4

N2 - Strains of human immunodeficiency virus type 1 differ in their abilities to infect and replicate in primary human macrophages. Chimeric clones were constructed from a provirus unable to infect macrophages (NLHX) and envelope sequences (V3 loop) of viruses derived without cultivation from brain (YU2 and w1-1c1) or spleen (w2-1b4) tissues. The substituted V3 loop sequences in each case were sufficient to confer upon NLHX the ability to infect macrophages. Furthermore, an envelope domain immediately N terminal to the V3 loop also was found to modulate the level of replication in macrophages. These results demonstrate that an envelope determinant derived directly from patients with AIDS confers HFV-1 tropism for macrophages.

AB - Strains of human immunodeficiency virus type 1 differ in their abilities to infect and replicate in primary human macrophages. Chimeric clones were constructed from a provirus unable to infect macrophages (NLHX) and envelope sequences (V3 loop) of viruses derived without cultivation from brain (YU2 and w1-1c1) or spleen (w2-1b4) tissues. The substituted V3 loop sequences in each case were sufficient to confer upon NLHX the ability to infect macrophages. Furthermore, an envelope domain immediately N terminal to the V3 loop also was found to modulate the level of replication in macrophages. These results demonstrate that an envelope determinant derived directly from patients with AIDS confers HFV-1 tropism for macrophages.

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Macrophage tropism determinants of human immunodeficiency virus type 1 in vivo

Abstract

ASJC Scopus subject areas

UN SDGs

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