Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis

Hsiao Ting Kuo, Jay Z. Yeh, Chi Ming Jiang, Ming Chang Wu*

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The objective of this study was to develop a magnetic particle-linked monoclonal antibody to hCG β for immunosorbent assay of human chorionic gonadotropin (hCG) with improved detection sensitivity. Monoclonal antibody against hCG β was found to be optimally cross-linked to the superparamagnetic particles (SPIO) using EDC and NHS as cross-linking reagents. This superparamagnetic particle-linked monoclonal antibody was able to concentrate hCG from a tested solution for further ELISA assay using horse radish peroxidase-labeled monoclonal antibody against hCG β. This hybrid technique had greatly decreased the detection limit to 0.1. mIU/mL, making an early detection of pregnancy possible. With an improved sensitivity and simple operation, the magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG) has a great potential to supersede the traditional ELISA for pregnancy diagnosis.

Original languageEnglish (US)
Pages (from-to)32-40
Number of pages9
JournalJournal of Immunological Methods
Volume381
Issue number1-2
DOIs
StatePublished - Jul 31 2012

Keywords

  • Human chorionic gonadotropin
  • Immunomagnetic particle-based ELISA
  • Magnetic particle-linked antibody immunoassay
  • Pregnancy diagnosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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