Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis

Hsiao Ting Kuo; Jay Z. Yeh; Chi Ming Jiang; Ming Chang Wu

doi:10.1016/j.jim.2012.04.006

Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis

Hsiao Ting Kuo, Jay Z. Yeh, Chi Ming Jiang, Ming Chang Wu^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The objective of this study was to develop a magnetic particle-linked monoclonal antibody to hCG β for immunosorbent assay of human chorionic gonadotropin (hCG) with improved detection sensitivity. Monoclonal antibody against hCG β was found to be optimally cross-linked to the superparamagnetic particles (SPIO) using EDC and NHS as cross-linking reagents. This superparamagnetic particle-linked monoclonal antibody was able to concentrate hCG from a tested solution for further ELISA assay using horse radish peroxidase-labeled monoclonal antibody against hCG β. This hybrid technique had greatly decreased the detection limit to 0.1. mIU/mL, making an early detection of pregnancy possible. With an improved sensitivity and simple operation, the magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG) has a great potential to supersede the traditional ELISA for pregnancy diagnosis.

Original language	English (US)
Pages (from-to)	32-40
Number of pages	9
Journal	Journal of Immunological Methods
Volume	381
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2012.04.006
State	Published - Jul 31 2012

Keywords

Human chorionic gonadotropin
Immunomagnetic particle-based ELISA
Magnetic particle-linked antibody immunoassay
Pregnancy diagnosis

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/j.jim.2012.04.006

Cite this

@article{66bce1ec2f764b16a3a2458f26354c73,

title = "Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis",

abstract = "The objective of this study was to develop a magnetic particle-linked monoclonal antibody to hCG β for immunosorbent assay of human chorionic gonadotropin (hCG) with improved detection sensitivity. Monoclonal antibody against hCG β was found to be optimally cross-linked to the superparamagnetic particles (SPIO) using EDC and NHS as cross-linking reagents. This superparamagnetic particle-linked monoclonal antibody was able to concentrate hCG from a tested solution for further ELISA assay using horse radish peroxidase-labeled monoclonal antibody against hCG β. This hybrid technique had greatly decreased the detection limit to 0.1. mIU/mL, making an early detection of pregnancy possible. With an improved sensitivity and simple operation, the magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG) has a great potential to supersede the traditional ELISA for pregnancy diagnosis.",

keywords = "Human chorionic gonadotropin, Immunomagnetic particle-based ELISA, Magnetic particle-linked antibody immunoassay, Pregnancy diagnosis",

author = "Kuo, {Hsiao Ting} and Yeh, {Jay Z.} and Jiang, {Chi Ming} and Wu, {Ming Chang}",

year = "2012",

month = jul,

day = "31",

doi = "10.1016/j.jim.2012.04.006",

language = "English (US)",

volume = "381",

pages = "32--40",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis

AU - Kuo, Hsiao Ting

AU - Yeh, Jay Z.

AU - Jiang, Chi Ming

AU - Wu, Ming Chang

PY - 2012/7/31

Y1 - 2012/7/31

N2 - The objective of this study was to develop a magnetic particle-linked monoclonal antibody to hCG β for immunosorbent assay of human chorionic gonadotropin (hCG) with improved detection sensitivity. Monoclonal antibody against hCG β was found to be optimally cross-linked to the superparamagnetic particles (SPIO) using EDC and NHS as cross-linking reagents. This superparamagnetic particle-linked monoclonal antibody was able to concentrate hCG from a tested solution for further ELISA assay using horse radish peroxidase-labeled monoclonal antibody against hCG β. This hybrid technique had greatly decreased the detection limit to 0.1. mIU/mL, making an early detection of pregnancy possible. With an improved sensitivity and simple operation, the magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG) has a great potential to supersede the traditional ELISA for pregnancy diagnosis.

AB - The objective of this study was to develop a magnetic particle-linked monoclonal antibody to hCG β for immunosorbent assay of human chorionic gonadotropin (hCG) with improved detection sensitivity. Monoclonal antibody against hCG β was found to be optimally cross-linked to the superparamagnetic particles (SPIO) using EDC and NHS as cross-linking reagents. This superparamagnetic particle-linked monoclonal antibody was able to concentrate hCG from a tested solution for further ELISA assay using horse radish peroxidase-labeled monoclonal antibody against hCG β. This hybrid technique had greatly decreased the detection limit to 0.1. mIU/mL, making an early detection of pregnancy possible. With an improved sensitivity and simple operation, the magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG) has a great potential to supersede the traditional ELISA for pregnancy diagnosis.

KW - Human chorionic gonadotropin

KW - Immunomagnetic particle-based ELISA

KW - Magnetic particle-linked antibody immunoassay

KW - Pregnancy diagnosis

UR - http://www.scopus.com/inward/record.url?scp=84861570767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861570767&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2012.04.006

DO - 10.1016/j.jim.2012.04.006

M3 - Article

C2 - 22542932

AN - SCOPUS:84861570767

SN - 0022-1759

VL - 381

SP - 32

EP - 40

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this