Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors

Alexander P. Glaser, Daniele Procissi, Yanni Yu, Joshua J Meeks

Research output: Contribution to journalArticle

Abstract

Murine bladder tumor models are critical for the evaluation of new therapeutic options. Bladder tumors induced with the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) carcinogen are advantageous over cell line-based models because they closely replicate the genomic profiles of human tumors, and, unlike cell models and xenografts, they provide a good opportunity for the study of immunotherapies. However, bladder tumor generation is heterogeneous; therefore, an accurate assessment of tumor burden is needed before randomization to experimental treatment. Described here is a BBN mouse model and protocol to evaluate bladder cancer tumor burden in vivo using a fast and reliable magnetic resonance (MR) sequence (true FISP). This method is simple and reliable because, unlike ultrasound, MR is operator-independent and allows for the straightforward post-acquisition image processing and review. Using axial images of the bladder, analysis of regions of interest along the bladder wall and tumor allow for the calculation of bladder wall and tumor area. This measurement correlates with ex vivo bladder weight (rs= 0.37, p = 0.009) and tumor stage (p = 0.0003). In conclusion, BBN generates heterogeneous tumors that are ideal for evaluation of immunotherapies, and MRI can quickly and reliably assess tumor burden prior to randomization to experimental treatment arms.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number145
DOIs
StatePublished - Mar 29 2019

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Carcinogens
Urinary Bladder Neoplasms
Tumors
Magnetic Resonance Imaging
Tumor Burden
Random Allocation
Immunotherapy
Urinary Bladder
Magnetic Resonance Spectroscopy
Butylhydroxybutylnitrosamine
Magnetic resonance
Neoplasms
Heterografts
Nitrosamines
Arm
Therapeutics
Weights and Measures
Cell Line
Magnetic resonance imaging
Image processing

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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title = "Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors",
abstract = "Murine bladder tumor models are critical for the evaluation of new therapeutic options. Bladder tumors induced with the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) carcinogen are advantageous over cell line-based models because they closely replicate the genomic profiles of human tumors, and, unlike cell models and xenografts, they provide a good opportunity for the study of immunotherapies. However, bladder tumor generation is heterogeneous; therefore, an accurate assessment of tumor burden is needed before randomization to experimental treatment. Described here is a BBN mouse model and protocol to evaluate bladder cancer tumor burden in vivo using a fast and reliable magnetic resonance (MR) sequence (true FISP). This method is simple and reliable because, unlike ultrasound, MR is operator-independent and allows for the straightforward post-acquisition image processing and review. Using axial images of the bladder, analysis of regions of interest along the bladder wall and tumor allow for the calculation of bladder wall and tumor area. This measurement correlates with ex vivo bladder weight (rs= 0.37, p = 0.009) and tumor stage (p = 0.0003). In conclusion, BBN generates heterogeneous tumors that are ideal for evaluation of immunotherapies, and MRI can quickly and reliably assess tumor burden prior to randomization to experimental treatment arms.",
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Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors. / Glaser, Alexander P.; Procissi, Daniele; Yu, Yanni; Meeks, Joshua J.

In: Journal of visualized experiments : JoVE, No. 145, 29.03.2019.

Research output: Contribution to journalArticle

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