Magnetization transfer imaging provides a quantitative measure of chondrogenic differentiation and tissue development

Weiguo Li, Liu Hong, Liping Hu, Richard L. Magin

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The goal of the present investigation was to test whether quantitative magnetization transfer imaging can be used as a noninvasive evaluation method for engineered cartilage. In this work, we used magnetic resonance imaging (MRI) to monitor the chondrogenesis of stem-cell-based engineered tissue over a 3-week period by measuring on a pixel-by-pixel basis the relaxation times (T1 and T2), the apparent diffusion coefficient, and the magnetization transfer parameters: bound proton fraction and cross-relaxation rate (k). Tissue-engineered constructs for generating cartilage were created by seeding mesenchymal stem cells in a gelatin sponge. Every 7 days, tissue samples were analyzed using MRI, histological, and biochemical methods. The MRI measurements were verified by histological analysis, and the imaging data were correlated with biochemical analysis of the developing cartilage matrix for glycosaminoglycan content. The MRI analysis for bound proton fraction and k showed a statistically significant increase that was correlated with the increase of glycosaminoglycan (R=0.96 and 0.87, respectively, p<0.05), whereas T1, T2, and apparent diffusion coefficient results did not show any significant changes over the 3-week measurement period.

Original languageEnglish (US)
Pages (from-to)1407-1415
Number of pages9
JournalTissue Engineering - Part C: Methods
Volume16
Issue number6
DOIs
StatePublished - Dec 1 2010

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

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