Abstract
Bladder cancer is one of the deadliest forms of cancer in modern medicine which despite recent progress has remained incurable and challenging for researchers. There is unmet need to address this endemic as the number of patients are growing by about 10,000 every year world-wide. Here, we report a minimally invasive magnetic chemotherapy method to address this problem where polyethylene glycol (PEG) functionalized Fe3O4 magnetic nanostructures (MNS) are homogeneously embedded in thermally responsive poly(N-isopropylacrylamide, NIPAAm) hydrogels (HG). The system (HG-MNS) loaded with anti-cancer drug doxorubicin (DOX) incubated with cancer cell lines subjected to external radiofrequency (RF) field can remotely stimulate the release of drug smartly at the site. The in vitro efficacy investigated on bladder cancer (T-24) cell lines showed the potential of the system in dealing with the disease successfully. Further, the materials preferential accumulation via systemic delivery was studied using swiss mice model. Vital tissue organs like liver, lung and heart were analysed by magnetic resonance imaging (MRI). A detail accounts of the materials optimization, cytotoxicity and anti-proliferation activity tests with apoptosis analysis by flow cytometry after RF exposure (250kHz) to the cells and in vivo biodistribution data are discussed in the paper.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 625-633 |
| Number of pages | 9 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 136 |
| DOIs | |
| State | Published - Dec 1 2015 |
Funding
This research was supported primarily by the Center of Cancer Nanotechnology Excellence (CCNE) initiative of the National Institutes of Health (NIH) under Award number U54CA151880. M.K.J. designed the experiments and prepared the manuscript. L.P. and S.V. helped in cell culture work and contributed to the editing of manuscript. M.D. helped in materials characterizations. H.D.S. and A.P. contributed in animal experiments. D.B. contributed to the novel idea and design of the project and helped prepared/edited the manuscript. V.P.D. supervised the entire project and helped in accomplishing the manuscript. Authors acknowledge the EPIC/NIFTI center for TEM/SEM and AFM imaging while CAMI at Northwestern for performing MRI. Authors are grateful to Animal House Facility at BARC, Mumbai, India for carrying out the in vivo work of the sample. M.K.J. acknowledges CCNE (Center of Nanotechnology Excellence) for providing financial assistance during stay at Northwestern University.
Keywords
- Bladder cancer
- Magnetic chemotherapy
- Magnetic resonance imaging
- Magneto-thermally responsive hydrogel
- PEG functionalized FeO
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
