MAGPIE/EGRET annotation of the 2.9-mb Drosophila melanogaster Adh region

Terry Gaasterland*, Alexander Sczyrba, Elizabeth Thomas, Gulriz Aytekin-Kurban, Paul Gordon, Christoph W. Sensen

*Corresponding author for this work

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Our challenge in annotating the 2.91-Mb Adh region of the Drosophila melanogaster genome was to identify genetic and genomic features automatically, completely, and precisely within a 6-week period. To do so, we augmented the MAGPIE microbial genome annotation system to handle eukaryotic genomic sequence data. The new configuration required the integration of eukaryotic gene-finding tools and DNA repeat tools into the automatic data collection module. It also required us to define in MAGPIE new strategies to combine data about eukaryotic exon predictions with functional data to refine the exon predictions. At the heart of the resulting new eukaryotic genome annotation system is a reverse comparison of public protein and complementary DNA sequences against the input genome to identify missing exons and to refine exon boundaries. The software modules that add eukaryotic genome annotation capability to MAGPIE are available as EGRET (Eukaryotic Genome Rapid Evaluation Tool).

Original languageEnglish (US)
Pages (from-to)502-510
Number of pages9
JournalGenome research
Volume10
Issue number4
DOIs
StatePublished - Apr 1 2000

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Gaasterland, T., Sczyrba, A., Thomas, E., Aytekin-Kurban, G., Gordon, P., & Sensen, C. W. (2000). MAGPIE/EGRET annotation of the 2.9-mb Drosophila melanogaster Adh region. Genome research, 10(4), 502-510. https://doi.org/10.1101/gr.10.4.502