TY - JOUR
T1 - Maintaining eastern newts (notophthalmus viridescens) for regeneration research
AU - Simon, Hans Georg
AU - Odelberg, Shannon
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015
PY - 2015
Y1 - 2015
N2 - The adult Eastern newt, Notophthalmus viridescens, has long served as a model for appendage as well as heart muscle regeneration studies. Newt tissues include all major cell types known in other vertebrates and mammals, including bone, cartilage, tendon, muscle, nerves, dermis, and epidermis. Therefore, these aquatic salamanders make an excellent model for studying the regeneration of complex tissues. Regeneration of adult tissues requires the integration of new tissues with preexisting tissues to form a functioning unit through a process that is not yet well understood. Scale is also an issue, because the regenerating tissues or structures are magnitudes larger than their embryonic counterparts during development, and therefore, it is likely that different physics and mechanics apply. Regardless, regeneration recapitulates to some degree developmental processes. In this chapter, we will describe basic methods for maintaining adult Eastern newts in the laboratory for the study of regeneration. To determine similarities and differences between development and regeneration at the cellular and molecular level, there is also a need for embryonic newt tissue. We therefore also outline a relatively simple way to produce and raise newt embryos in the laboratory.
AB - The adult Eastern newt, Notophthalmus viridescens, has long served as a model for appendage as well as heart muscle regeneration studies. Newt tissues include all major cell types known in other vertebrates and mammals, including bone, cartilage, tendon, muscle, nerves, dermis, and epidermis. Therefore, these aquatic salamanders make an excellent model for studying the regeneration of complex tissues. Regeneration of adult tissues requires the integration of new tissues with preexisting tissues to form a functioning unit through a process that is not yet well understood. Scale is also an issue, because the regenerating tissues or structures are magnitudes larger than their embryonic counterparts during development, and therefore, it is likely that different physics and mechanics apply. Regardless, regeneration recapitulates to some degree developmental processes. In this chapter, we will describe basic methods for maintaining adult Eastern newts in the laboratory for the study of regeneration. To determine similarities and differences between development and regeneration at the cellular and molecular level, there is also a need for embryonic newt tissue. We therefore also outline a relatively simple way to produce and raise newt embryos in the laboratory.
KW - Breeding
KW - Eastern newt
KW - Embryo
KW - Larva
KW - Notophthalmus viridescens
KW - Red eft
KW - Red-spotted newt
KW - Regeneration
KW - Spawning
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U2 - 10.1007/978-1-4939-2495-0_2
DO - 10.1007/978-1-4939-2495-0_2
M3 - Article
C2 - 25740474
AN - SCOPUS:84924576748
SN - 1064-3745
VL - 1290
SP - 17
EP - 25
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -