Maitotoxin‐Induced Release of γ‐[3H]Aminobutyric Acid from Cultures of Striatal Neurons

Ismail A. Shalaby, Sathapana Kongsamut, Richard J. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Abstract: The potent marine toxin, maitotoxin, induced the release of γ‐[3H]aminobutyric acid (GABA) from reaggregate cultures of striatal neurons in a dose‐dependent manner. Maitotoxin‐induced release occurred following a lag period of several minutes and was persistent. Release induced by 70 mM K+ on the other hand was immediate and transient in nature. Co2+ (3 mM) and Cd2+ (1 mM) inhibited maitotoxin‐induced release of GABA as did removal of extracellular Ca2+. However, the organic calcium antagonists nisoldipine, nitrendipine, and D‐600 at concentrations of 10−6M did not block maitotoxin‐induced or 70 mM K+‐induced release. High concentrations of D‐600 (10−4M) partially blocked both maitotoxin‐ and 70 mM K+‐induced release. The dihydropyridine calcium agonist BAY K8644 (10−6M) did not enhance maitotoxin‐induced or 70 mM K+‐induced release. Replacement of Na+ in the incubation medium with choline led to an increased basal output of GABA and an apparent inhibition of the effect of maitotoxin. These data are discussed with reference to the hypothesis that maitotoxin can directly activate voltage‐sensitive calcium channels.

Original languageEnglish (US)
Pages (from-to)1161-1165
Number of pages5
JournalJournal of Neurochemistry
Volume46
Issue number4
DOIs
StatePublished - Jan 1 1986

Keywords

  • Maitotoxin
  • Neurons
  • Striatum
  • Transmitter release
  • γ‐Aminobutyric acid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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