Making live-cell imaging glow

Margaret L. Gardel, Yvonne S. Aratyn, Thomas J. Hope, Robert Nowak, Andrew DeSimone, Linda Smith

Research output: Contribution to specialist publicationArticle

Abstract

Digital illumination, in combination with confocal and wide-field microscopy promises to overcome the potential of fluorescence imaging of live-cell imaging. Fluorescence recovery after photobleaching (FRAP) and photoactivation experiments targeting multiple regions of interest (ROI) are employed to identify and analyze the mobility of proteins incorporated within sub-cellular structures. Digital illumination allows to make observations and to validate cell migration data, while fluorescence imaging is achieving sufficient excitation energy while maintaining the integrity of the sample and fluorophore. Digital micromirror device (DMD) holds the promise of revolutionizing live-cell imaging and is poised to do the same for other fluorescence imaging applications. Researchers can simultaneously and accurately excite fluorophores in multiple regions of interest with complex geometries and with zero delta time.

Original languageEnglish (US)
Pages24-27
Number of pages4
Volume15
No11
Specialist publicationBiophotonics International
StatePublished - Nov 1 2008

ASJC Scopus subject areas

  • Biotechnology

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