Making live-cell imaging glow

Margaret L. Gardel; Yvonne S. Aratyn; Thomas J. Hope; Robert Nowak; Andrew DeSimone; Linda Smith

Making live-cell imaging glow

Margaret L. Gardel, Yvonne S. Aratyn, Thomas J. Hope, Robert Nowak, Andrew DeSimone, Linda Smith

Cell & Developmental Biology

Research output: Contribution to specialist publication › Article

Abstract

Digital illumination, in combination with confocal and wide-field microscopy promises to overcome the potential of fluorescence imaging of live-cell imaging. Fluorescence recovery after photobleaching (FRAP) and photoactivation experiments targeting multiple regions of interest (ROI) are employed to identify and analyze the mobility of proteins incorporated within sub-cellular structures. Digital illumination allows to make observations and to validate cell migration data, while fluorescence imaging is achieving sufficient excitation energy while maintaining the integrity of the sample and fluorophore. Digital micromirror device (DMD) holds the promise of revolutionizing live-cell imaging and is poised to do the same for other fluorescence imaging applications. Researchers can simultaneously and accurately excite fluorophores in multiple regions of interest with complex geometries and with zero delta time.

Original language	English (US)
Pages	24-27
Number of pages	4
Volume	15
No	11
Specialist publication	Biophotonics International
State	Published - Nov 1 2008

ASJC Scopus subject areas

Biotechnology

Cite this

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title = "Making live-cell imaging glow",

abstract = "Digital illumination, in combination with confocal and wide-field microscopy promises to overcome the potential of fluorescence imaging of live-cell imaging. Fluorescence recovery after photobleaching (FRAP) and photoactivation experiments targeting multiple regions of interest (ROI) are employed to identify and analyze the mobility of proteins incorporated within sub-cellular structures. Digital illumination allows to make observations and to validate cell migration data, while fluorescence imaging is achieving sufficient excitation energy while maintaining the integrity of the sample and fluorophore. Digital micromirror device (DMD) holds the promise of revolutionizing live-cell imaging and is poised to do the same for other fluorescence imaging applications. Researchers can simultaneously and accurately excite fluorophores in multiple regions of interest with complex geometries and with zero delta time.",

author = "Gardel, {Margaret L.} and Aratyn, {Yvonne S.} and Hope, {Thomas J.} and Robert Nowak and Andrew DeSimone and Linda Smith",

year = "2008",

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language = "English (US)",

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AU - Gardel, Margaret L.

AU - Aratyn, Yvonne S.

AU - Hope, Thomas J.

AU - Nowak, Robert

AU - DeSimone, Andrew

AU - Smith, Linda

PY - 2008/11/1

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N2 - Digital illumination, in combination with confocal and wide-field microscopy promises to overcome the potential of fluorescence imaging of live-cell imaging. Fluorescence recovery after photobleaching (FRAP) and photoactivation experiments targeting multiple regions of interest (ROI) are employed to identify and analyze the mobility of proteins incorporated within sub-cellular structures. Digital illumination allows to make observations and to validate cell migration data, while fluorescence imaging is achieving sufficient excitation energy while maintaining the integrity of the sample and fluorophore. Digital micromirror device (DMD) holds the promise of revolutionizing live-cell imaging and is poised to do the same for other fluorescence imaging applications. Researchers can simultaneously and accurately excite fluorophores in multiple regions of interest with complex geometries and with zero delta time.

AB - Digital illumination, in combination with confocal and wide-field microscopy promises to overcome the potential of fluorescence imaging of live-cell imaging. Fluorescence recovery after photobleaching (FRAP) and photoactivation experiments targeting multiple regions of interest (ROI) are employed to identify and analyze the mobility of proteins incorporated within sub-cellular structures. Digital illumination allows to make observations and to validate cell migration data, while fluorescence imaging is achieving sufficient excitation energy while maintaining the integrity of the sample and fluorophore. Digital micromirror device (DMD) holds the promise of revolutionizing live-cell imaging and is poised to do the same for other fluorescence imaging applications. Researchers can simultaneously and accurately excite fluorophores in multiple regions of interest with complex geometries and with zero delta time.

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Making live-cell imaging glow

Abstract

ASJC Scopus subject areas

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