Digital illumination, in combination with confocal and wide-field microscopy promises to overcome the potential of fluorescence imaging of live-cell imaging. Fluorescence recovery after photobleaching (FRAP) and photoactivation experiments targeting multiple regions of interest (ROI) are employed to identify and analyze the mobility of proteins incorporated within sub-cellular structures. Digital illumination allows to make observations and to validate cell migration data, while fluorescence imaging is achieving sufficient excitation energy while maintaining the integrity of the sample and fluorophore. Digital micromirror device (DMD) holds the promise of revolutionizing live-cell imaging and is poised to do the same for other fluorescence imaging applications. Researchers can simultaneously and accurately excite fluorophores in multiple regions of interest with complex geometries and with zero delta time.
|Original language||English (US)|
|Number of pages||4|
|Specialist publication||Biophotonics International|
|State||Published - Nov 1 2008|
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