Conformational differences between manganese hemoglobin and its liganded derivatives are observed in the tetramer-dimer dissociation equilibrium, in the binding of 8-hydroxy-1,3,6-pyrenetrisulfonate (an analogue of 2,3-diphosphoglycerate) and bromthymol blue, and in the reaction with p-hydroxymercuribenzoate. The reaction of manganese hemoglobin with NO is readily followed by stopped flow, the resulting NO derivative is photodissociable, and rapid kinetic measurements exhibit the hemoglobin allosteric interaction.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 10 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology