Manganese hemoglobin: Allosteric effects in stopped flow flash photolysis and sedimentation measurements

Q. H. Gibson*, B. M. Hoffman, R. H. Crepeau, S. J. Edelstein, C. Bull

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Conformational differences between manganese hemoglobin and its liganded derivatives are observed in the tetramer-dimer dissociation equilibrium, in the binding of 8-hydroxy-1,3,6-pyrenetrisulfonate (an analogue of 2,3-diphosphoglycerate) and bromthymol blue, and in the reaction with p-hydroxymercuribenzoate. The reaction of manganese hemoglobin with NO is readily followed by stopped flow, the resulting NO derivative is photodissociable, and rapid kinetic measurements exhibit the hemoglobin allosteric interaction.

Original languageEnglish (US)
Pages (from-to)146-151
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume59
Issue number1
DOIs
StatePublished - Jul 10 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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