Manganese hemoglobin: Allosteric effects in stopped flow flash photolysis and sedimentation measurements

Q. H. Gibson; B. M. Hoffman; R. H. Crepeau; S. J. Edelstein; C. Bull

doi:10.1016/S0006-291X(74)80186-5

Manganese hemoglobin: Allosteric effects in stopped flow flash photolysis and sedimentation measurements

Q. H. Gibson^*, B. M. Hoffman, R. H. Crepeau, S. J. Edelstein, C. Bull

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Conformational differences between manganese hemoglobin and its liganded derivatives are observed in the tetramer-dimer dissociation equilibrium, in the binding of 8-hydroxy-1,3,6-pyrenetrisulfonate (an analogue of 2,3-diphosphoglycerate) and bromthymol blue, and in the reaction with p-hydroxymercuribenzoate. The reaction of manganese hemoglobin with NO is readily followed by stopped flow, the resulting NO derivative is photodissociable, and rapid kinetic measurements exhibit the hemoglobin allosteric interaction.

Original language	English (US)
Pages (from-to)	146-151
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(74)80186-5
State	Published - Jul 10 1974

Funding

Acknowledgments: We are indebted to Anne Griffin for technical assistance. C.B. is a predoctoral trainee of the USPHS, administered by the National Institutes Institutes of Health, training grant GM00626. This work was supported by the National Institutes of Health, Grants HL-135~I (BMH), GM-14276-08 (QHG)~ and HL-13591 (SJE) and by the National Science Foundation~ Grant GB8773 (SJE).

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Manganese hemoglobin: Allosteric effects in stopped flow flash photolysis and sedimentation measurements",

abstract = "Conformational differences between manganese hemoglobin and its liganded derivatives are observed in the tetramer-dimer dissociation equilibrium, in the binding of 8-hydroxy-1,3,6-pyrenetrisulfonate (an analogue of 2,3-diphosphoglycerate) and bromthymol blue, and in the reaction with p-hydroxymercuribenzoate. The reaction of manganese hemoglobin with NO is readily followed by stopped flow, the resulting NO derivative is photodissociable, and rapid kinetic measurements exhibit the hemoglobin allosteric interaction.",

author = "Gibson, {Q. H.} and Hoffman, {B. M.} and Crepeau, {R. H.} and Edelstein, {S. J.} and C. Bull",

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AU - Gibson, Q. H.

AU - Hoffman, B. M.

AU - Crepeau, R. H.

AU - Edelstein, S. J.

AU - Bull, C.

N1 - Funding Information: Acknowledgments: We are indebted to Anne Griffin for technical assistance. C.B. is a predoctoral trainee of the USPHS, administered by the National Institutes Institutes of Health, training grant GM00626. This work was supported by the National Institutes of Health, Grants HL-135~I (BMH), GM-14276-08 (QHG)~ and HL-13591 (SJE) and by the National Science Foundation~ Grant GB8773 (SJE).

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Y1 - 1974/7/10

N2 - Conformational differences between manganese hemoglobin and its liganded derivatives are observed in the tetramer-dimer dissociation equilibrium, in the binding of 8-hydroxy-1,3,6-pyrenetrisulfonate (an analogue of 2,3-diphosphoglycerate) and bromthymol blue, and in the reaction with p-hydroxymercuribenzoate. The reaction of manganese hemoglobin with NO is readily followed by stopped flow, the resulting NO derivative is photodissociable, and rapid kinetic measurements exhibit the hemoglobin allosteric interaction.

AB - Conformational differences between manganese hemoglobin and its liganded derivatives are observed in the tetramer-dimer dissociation equilibrium, in the binding of 8-hydroxy-1,3,6-pyrenetrisulfonate (an analogue of 2,3-diphosphoglycerate) and bromthymol blue, and in the reaction with p-hydroxymercuribenzoate. The reaction of manganese hemoglobin with NO is readily followed by stopped flow, the resulting NO derivative is photodissociable, and rapid kinetic measurements exhibit the hemoglobin allosteric interaction.

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Manganese hemoglobin: Allosteric effects in stopped flow flash photolysis and sedimentation measurements

Abstract

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