Manipulating immune activation of macrophages by tuning the oligonucleotide composition of gold nanoparticles

Roger M. Pallares, Priscilla Choo, Lisa E. Cole, Chad A Mirkin, Andrew Lee, Teri W Odom*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

This paper describes how the ligand shell containing immunostimulatory oligonucleotides surrounding gold nanoparticles affects the in vitro activation of macrophages. Nanoconstructs with similar ligand densities but different oligonucleotide compositions (from 0% to 100% immune-active cytosine-phosphate-guanine, CpG) were compared. Maximum immunostimulation was achieved with CpG content as low as 5% (with total oligonucleotide surface coverage remaining constant), correlating to high levels of antitumor cytokine release and low levels of cancer-promoting ones. Independent of CpG content, gold nanoparticles with low oligonucleotide densities exhibit poor cellular uptake, leading to insignificant immunostimulation and cytokine release. By identifying effects of ligand shell composition on macrophage activation, we can inform the design rules of therapeutic nanoconstructs to achieve specific immune responses.

Original languageEnglish (US)
Pages (from-to)2032-2037
Number of pages6
JournalBioconjugate Chemistry
Volume30
Issue number7
DOIs
StatePublished - Jul 17 2019

Fingerprint

Macrophage Activation
Macrophages
Oligonucleotides
Gold
Nanoparticles
Tuning
Chemical activation
Ligands
Chemical analysis
Immunization
Cytokines
Cytosine
Guanine
Phosphates
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

@article{035718a9cf5c432fb38c73c3a7ef8767,
title = "Manipulating immune activation of macrophages by tuning the oligonucleotide composition of gold nanoparticles",
abstract = "This paper describes how the ligand shell containing immunostimulatory oligonucleotides surrounding gold nanoparticles affects the in vitro activation of macrophages. Nanoconstructs with similar ligand densities but different oligonucleotide compositions (from 0{\%} to 100{\%} immune-active cytosine-phosphate-guanine, CpG) were compared. Maximum immunostimulation was achieved with CpG content as low as 5{\%} (with total oligonucleotide surface coverage remaining constant), correlating to high levels of antitumor cytokine release and low levels of cancer-promoting ones. Independent of CpG content, gold nanoparticles with low oligonucleotide densities exhibit poor cellular uptake, leading to insignificant immunostimulation and cytokine release. By identifying effects of ligand shell composition on macrophage activation, we can inform the design rules of therapeutic nanoconstructs to achieve specific immune responses.",
author = "Pallares, {Roger M.} and Priscilla Choo and Cole, {Lisa E.} and Mirkin, {Chad A} and Andrew Lee and Odom, {Teri W}",
year = "2019",
month = "7",
day = "17",
doi = "10.1021/acs.bioconjchem.9b00316",
language = "English (US)",
volume = "30",
pages = "2032--2037",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "7",

}

Manipulating immune activation of macrophages by tuning the oligonucleotide composition of gold nanoparticles. / Pallares, Roger M.; Choo, Priscilla; Cole, Lisa E.; Mirkin, Chad A; Lee, Andrew; Odom, Teri W.

In: Bioconjugate Chemistry, Vol. 30, No. 7, 17.07.2019, p. 2032-2037.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Manipulating immune activation of macrophages by tuning the oligonucleotide composition of gold nanoparticles

AU - Pallares, Roger M.

AU - Choo, Priscilla

AU - Cole, Lisa E.

AU - Mirkin, Chad A

AU - Lee, Andrew

AU - Odom, Teri W

PY - 2019/7/17

Y1 - 2019/7/17

N2 - This paper describes how the ligand shell containing immunostimulatory oligonucleotides surrounding gold nanoparticles affects the in vitro activation of macrophages. Nanoconstructs with similar ligand densities but different oligonucleotide compositions (from 0% to 100% immune-active cytosine-phosphate-guanine, CpG) were compared. Maximum immunostimulation was achieved with CpG content as low as 5% (with total oligonucleotide surface coverage remaining constant), correlating to high levels of antitumor cytokine release and low levels of cancer-promoting ones. Independent of CpG content, gold nanoparticles with low oligonucleotide densities exhibit poor cellular uptake, leading to insignificant immunostimulation and cytokine release. By identifying effects of ligand shell composition on macrophage activation, we can inform the design rules of therapeutic nanoconstructs to achieve specific immune responses.

AB - This paper describes how the ligand shell containing immunostimulatory oligonucleotides surrounding gold nanoparticles affects the in vitro activation of macrophages. Nanoconstructs with similar ligand densities but different oligonucleotide compositions (from 0% to 100% immune-active cytosine-phosphate-guanine, CpG) were compared. Maximum immunostimulation was achieved with CpG content as low as 5% (with total oligonucleotide surface coverage remaining constant), correlating to high levels of antitumor cytokine release and low levels of cancer-promoting ones. Independent of CpG content, gold nanoparticles with low oligonucleotide densities exhibit poor cellular uptake, leading to insignificant immunostimulation and cytokine release. By identifying effects of ligand shell composition on macrophage activation, we can inform the design rules of therapeutic nanoconstructs to achieve specific immune responses.

UR - http://www.scopus.com/inward/record.url?scp=85068145534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068145534&partnerID=8YFLogxK

U2 - 10.1021/acs.bioconjchem.9b00316

DO - 10.1021/acs.bioconjchem.9b00316

M3 - Article

VL - 30

SP - 2032

EP - 2037

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 7

ER -