Mannose‐sensitive haemagglutination in the absence of piliation in Escherichia coli

S. J. Hultgren*, J. L. Duncan, A. J. Schaeffer, S. K. Amundsen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The relationship between type 1 pilus structure and the mannose‐sensitive adhesin was investigated by analysing the properties of an 11.2 kb fragment of DNA derived from the chromosomal pil region of a type 1 piliated uropathogenic strain of Escherichia coli. The recombinant plasmids pHA9 and pSJH9, containing the cloned fragment, conferred a mannose‐sensitive haemagglutination (MSHA)‐positive but non‐piliated phenotype on recipient cells of ORN104. Most of the DNA sequences homologous to the pilA and hyp genes were not present in the 11.2kb insert, and the genetic information necessary for MSHA in the absence of piliation spanned a 6.5 kb region of the cloned fragment. The polypeptides expressed by pSJH9 were examined in minicells and Tn 1000 insertions in three genes encoding proteins of molecular weights 90 kD, 29 kD and 17kD abolished the MSHA phenotype.

Original languageEnglish (US)
Pages (from-to)1311-1318
Number of pages8
JournalMolecular Microbiology
Volume4
Issue number8
DOIs
StatePublished - Aug 1990

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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