Mapping DNA and protein in biological samples using the scanning transmission x-ray microscope

X. Zhang*, R. Balhorn, C. Jacobsen, J. Kirz, S. Williams

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The Scanning Transmission soft X-ray Microscope (STXM) at the X1A beamline at the National Synchrotron Light Source, Brookhaven National Library has been used to image wet biological samples using the natural absorption differences between carbon and water in the water window. The step-like jumps in the absorption of soft x-rays by materials as a function of energy have been used for elemental mapping. The paper examines the x-ray absorption fine structure spectra at the carbon absorption edge from DNA and bovine serum albumin taken using the STXM. Differences between the spectra of these two biologically important molecules can be used to distinguish DNA and proteins.

Original languageEnglish (US)
Title of host publicationProceedings - Annual Meeting, Microscopy Society of America
EditorsG.W. Bailey, A.J. Garratt-Reed
Pages50-51
Number of pages2
StatePublished - Dec 1 1994
EventProceedings of the 52nd Annual Meeting of the Microscopy Society of America - New Orleans, LA, USA
Duration: Jul 31 1994Aug 5 1994

Other

OtherProceedings of the 52nd Annual Meeting of the Microscopy Society of America
CityNew Orleans, LA, USA
Period7/31/948/5/94

ASJC Scopus subject areas

  • Engineering(all)

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