Mapping of ER gene CpG island methylation by methylation-specific polymerase chain reaction

Rena G. Lapidus; Sharyl J. Nass; Kimberly A. Butash; Fritz F. Parl; Sigmund A. Weitzman; Jeremy G. Graff; James G. Herman; Nancy E. Davidson

Mapping of ER gene CpG island methylation by methylation-specific polymerase chain reaction

Rena G. Lapidus, Sharyl J. Nass, Kimberly A. Butash, Fritz F. Parl, Sigmund A. Weitzman, Jeremy G. Graff, James G. Herman, Nancy E. Davidson^*

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

206 Scopus citations

Abstract

Southern analysis has shown that DNA from 25% of primary estrogen receptor (ER) α -negative breast tumors displays aberrant methylation at one site within the ER gene CpG island. To examine more sites and increase sensitivity, we developed a methylation-specific PCR assay to map methylation of the entire ER CpG island. The island was unmethylated in normal breast tissue and ER-positive breast cancer cell lines, but extensively methylated in all ER-negative cell lines and breast tumors examined. In addition, some of the ER-positive/progesterone receptor-negative and ER- positive/progesterone receptor-positive tumors (about 70% and 35%, respectively) displayed methylation of the ER CpG island, suggesting that this heterogeneity within tumor cell populations could potentially shed light on the etiology of ER-negative recurrent tumors arising from ER-positive tumors.

Original language	English (US)
Pages (from-to)	2515-2519
Number of pages	5
Journal	Cancer Research
Volume	58
Issue number	12
State	Published - Jun 15 1998

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Mapping of ER gene CpG island methylation by methylation-specific polymerase chain reaction",

abstract = "Southern analysis has shown that DNA from 25% of primary estrogen receptor (ER) α -negative breast tumors displays aberrant methylation at one site within the ER gene CpG island. To examine more sites and increase sensitivity, we developed a methylation-specific PCR assay to map methylation of the entire ER CpG island. The island was unmethylated in normal breast tissue and ER-positive breast cancer cell lines, but extensively methylated in all ER-negative cell lines and breast tumors examined. In addition, some of the ER-positive/progesterone receptor-negative and ER- positive/progesterone receptor-positive tumors (about 70% and 35%, respectively) displayed methylation of the ER CpG island, suggesting that this heterogeneity within tumor cell populations could potentially shed light on the etiology of ER-negative recurrent tumors arising from ER-positive tumors.",

author = "Lapidus, {Rena G.} and Nass, {Sharyl J.} and Butash, {Kimberly A.} and Parl, {Fritz F.} and Weitzman, {Sigmund A.} and Graff, {Jeremy G.} and Herman, {James G.} and Davidson, {Nancy E.}",

year = "1998",

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language = "English (US)",

volume = "58",

pages = "2515--2519",

journal = "Cancer Research",

issn = "0008-5472",

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T1 - Mapping of ER gene CpG island methylation by methylation-specific polymerase chain reaction

AU - Lapidus, Rena G.

AU - Nass, Sharyl J.

AU - Butash, Kimberly A.

AU - Parl, Fritz F.

AU - Weitzman, Sigmund A.

AU - Graff, Jeremy G.

AU - Herman, James G.

AU - Davidson, Nancy E.

PY - 1998/6/15

Y1 - 1998/6/15

N2 - Southern analysis has shown that DNA from 25% of primary estrogen receptor (ER) α -negative breast tumors displays aberrant methylation at one site within the ER gene CpG island. To examine more sites and increase sensitivity, we developed a methylation-specific PCR assay to map methylation of the entire ER CpG island. The island was unmethylated in normal breast tissue and ER-positive breast cancer cell lines, but extensively methylated in all ER-negative cell lines and breast tumors examined. In addition, some of the ER-positive/progesterone receptor-negative and ER- positive/progesterone receptor-positive tumors (about 70% and 35%, respectively) displayed methylation of the ER CpG island, suggesting that this heterogeneity within tumor cell populations could potentially shed light on the etiology of ER-negative recurrent tumors arising from ER-positive tumors.

AB - Southern analysis has shown that DNA from 25% of primary estrogen receptor (ER) α -negative breast tumors displays aberrant methylation at one site within the ER gene CpG island. To examine more sites and increase sensitivity, we developed a methylation-specific PCR assay to map methylation of the entire ER CpG island. The island was unmethylated in normal breast tissue and ER-positive breast cancer cell lines, but extensively methylated in all ER-negative cell lines and breast tumors examined. In addition, some of the ER-positive/progesterone receptor-negative and ER- positive/progesterone receptor-positive tumors (about 70% and 35%, respectively) displayed methylation of the ER CpG island, suggesting that this heterogeneity within tumor cell populations could potentially shed light on the etiology of ER-negative recurrent tumors arising from ER-positive tumors.

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JF - Cancer Research

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ER -

Mapping of ER gene CpG island methylation by methylation-specific polymerase chain reaction

Abstract

ASJC Scopus subject areas

UN SDGs

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