Mapping of the gene encoding human β-defensin-2 (DEFB2) to chromosome region 8p22-p23.1

Jürgen Harder, Reiner Siebert, Yanming Zhang, Peter Matthiesen, Enno Christophers, Brigitte Schlegelberger, Jens M. Schröder*

*Corresponding author for this work

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

We recently reported the isolation of human β-defensin-2 (hBD-2), a novel epithelia-derived peptide antibiotic belonging to the β-defensin family. hBD-2 is expressed in skin and epithelia of the airway system, where it is believed to contribute to its antimicrobial defense. By fluorescence in situ hybridization using a hBD-2 genomic DNA probe and subsequent fluorescence R-banding, the hBD-2 gene (HGMW-approved symbol DEFB2) was assigned to human chromosome region 8p22-p23.1. PCR with a set of CEPH YAC clones spanning this chromosomal region revealed CEPH YACs 773G4, 920D12, and 820B4 to contain the hBD-2 gene. Relying on the preexisting physical maps of 8p22-p23.1, the hBD-2 gene was mapped in close proximity to D8S1993 (WI- 9956) within the interval flanked by D8S552 and D8S1130 (CHLC.GATA25C10). The fact that all currently described genes encoding defensins map to chromosome 8p21-pter suggests that a gene cluster in this chromosomal region may play a major role in antimicrobial defense.

Original languageEnglish (US)
Pages (from-to)472-475
Number of pages4
JournalGenomics
Volume46
Issue number3
DOIs
StatePublished - Dec 15 1997

ASJC Scopus subject areas

  • Genetics

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    Harder, J., Siebert, R., Zhang, Y., Matthiesen, P., Christophers, E., Schlegelberger, B., & Schröder, J. M. (1997). Mapping of the gene encoding human β-defensin-2 (DEFB2) to chromosome region 8p22-p23.1. Genomics, 46(3), 472-475. https://doi.org/10.1006/geno.1997.5074