Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI

Takashi Watanabe*, Jelena Radulovic, Susann Boretius, Jens Frahm, Thomas Michaelis

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

This magnetic resonance imaging (MRI) study describes mapping of the habenulo-interpeduncular pathway in living mice based on manganese-induced contrast. Six hours after intracerebroventricular microinjection of MnCl 2, T1-weighted 3D MRI (2.35 T) at 117 μm isotropic resolution revealed a continuous pattern of anterograde labeling from the habenula via the fasciculus retroflexus to the interpeduncular nucleus. Alternatively, the less invasive systemic administration of MnCl2 allowed for monitoring of the dynamic uptake pattern of respective neural components with even higher reproducibility across animals. Time courses covered the range from 42 min to 24 h after injection. In conclusion, manganese-enhanced MRI may open new ways for functional assessments of the habenulo-interpeduncular system in animal models with cognitive impairment.

Original languageEnglish (US)
Pages (from-to)209-215
Number of pages7
JournalMagnetic Resonance Imaging
Volume24
Issue number3
DOIs
StatePublished - Apr 1 2006

Keywords

  • Habenula
  • Magnetic resonance imaging
  • Manganese
  • Mice
  • Neural pathways

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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