Mapping projections of molecularly defined dopamine neuron subtypes using intersectional genetic approaches

Jean Francois Poulin, Giuliana Caronia, Caitlyn Hofer, Qiaoling Cui, Brandon Helm, Charu Ramakrishnan, C. Savio Chan, Daniel A. Dombeck, Karl Deisseroth, Rajeshwar Awatramani*

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Midbrain dopamine (DA) neurons have diverse functions that can in part be explained by their heterogeneity. Although molecularly distinct subtypes of DA neurons have been identified by single-cell gene expression profiling, fundamental features such as their projection patterns have not been elucidated. Progress in this regard has been hindered by the lack of genetic tools for studying DA neuron subtypes. Here we develop intersectional genetic labeling strategies, based on combinatorial gene expression, to map the projections of molecularly defined DA neuron subtypes. We reveal distinct genetically defined dopaminergic pathways arising from the substantia nigra pars compacta and from the ventral tegmental area that innervate specific regions of the caudate putamen, nucleus accumbens and amygdala. Together, the genetic toolbox and DA neuron subtype projections presented here constitute a resource that will accelerate the investigation of this clinically significant neurotransmitter system.

Original languageEnglish (US)
Pages (from-to)1260-1271
Number of pages12
JournalNature Neuroscience
Volume21
Issue number9
DOIs
StatePublished - Sep 1 2018

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ASJC Scopus subject areas

  • Neuroscience(all)

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